WormBase Tree Display for Variation: WBVar00088906
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WBVar00088906 | Name | Public_name | mgDf90 | |||||
---|---|---|---|---|---|---|---|---|
Other_name | mg90 | |||||||
Sequence_details | SeqStatus | Pending_curation | ||||||
Variation_type | Allele | |||||||
Origin | Species | Caenorhabditis elegans | ||||||
Laboratory | GR | |||||||
Status | Live | |||||||
Affects | Gene | WBGene00000899 | ||||||
Interactor (17) | ||||||||
Description | Phenotype | WBPhenotype:0000061 | Paper_evidence | WBPaper00038374 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "When compared to the daf-2(e1370) parental strain, daf-2(e1370); daf-3(mgDf90) mutants lived significantly longer. This was also observed in daf-2(e1370); daf-3(e1376) worms, which is a weaker allele of daf-3." (see Figure 4A) | Paper_evidence | WBPaper00038374 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | daf-2(e1370) | Paper_evidence | WBPaper00038374 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000135 | Paper_evidence | WBPaper00054590 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Genotype | daf-3(mgDF90)X; muIs84[pAD76(psod-3::gfp)]; muEx541[pPC30(pgpa-4::GFP::Tetx) +punc-122::RFP] | Paper_evidence | WBPaper00054590 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000136 | Paper_evidence | WBPaper00038374 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "We tested changes in the levels of these insulins using Q-PCR in TGFb pathway mutants such as daf-3(mgDf90), daf-14(m77) as well as pdp-1(tm3734) and compared them to wild-type worms (Figure 5A-5C, Figure S14, Tables S2 and S3). Interestingly, both pdp-1(tm3734) and daf-3(mgDf90) showed elevated levels of several insulins as compared to wild-type worms (Figure 5A and Figure S14). In contrast, expression of these insulins was markedly reduced in daf-14(m77) mutants (Figure 5B and Figure S14)." | Paper_evidence | WBPaper00038374 | |||||
Curator_confirmed | WBPerson2987 | |||||||
daf-3(mgDf90) results in increased levels of pdp-1 mRNA, as determined by qRT-PCR (Figure S15) | Paper_evidence | WBPaper00038374 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000637 | Paper_evidence | WBPaper00038374 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "daf-2(e1370); daf-3(mgDf90) worms show significant enhancement of daf-2(e1370) dauer formation across several temperatures tested, whereas a daf-5 mutation or daf-5 RNAi results in reduced daf-2(e1370) dauer formation (Figure 4Ci, Figure 4Cii and Figure S11)." | Paper_evidence | WBPaper00038374 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | daf-2(e1370) | Paper_evidence | WBPaper00038374 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001090 | Paper_evidence | WBPaper00038374 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Figure S13C | Paper_evidence | WBPaper00038374 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001171 | Paper_evidence | WBPaper00038374 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Table S1 | Paper_evidence | WBPaper00038374 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001655 | Paper_evidence | WBPaper00026674 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Table 3 | Paper_evidence | WBPaper00026674 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Affected_by | Molecule | WBMol:00003022 | Paper_evidence | WBPaper00026674 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_not_observed | WBPhenotype:0001182 | Paper_evidence | WBPaper00038374 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "We next tested the role of DAF-3 and DAF-5 on fat storage, dauer formation and stress resistance. Oil Red O staining for fat storage showed comparable levels between daf-2(e1370) and daf-2(e1370); daf-3(mgDf90) worms, but markedly lesser amounts of fat in daf-5(e1386); daf-2(e1370) worms (Figure 4B top and bottom panel and Figure S12)." | Paper_evidence | WBPaper00038374 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | daf-2(e1370) | Paper_evidence | WBPaper00038374 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Reference | WBPaper00038374 | |||||||
WBPaper00026674 | ||||||||
WBPaper00011034 | ||||||||
WBPaper00017169 | ||||||||
WBPaper00054590 | ||||||||
Remark | This object is now known to be a deficiency and has been reassigned a Rearrangement object mgDf90 | Person_evidence | WBPerson545 | |||||
Curator_confirmed | WBPerson2970 | |||||||
Method | Allele |