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WormBase Tree Display for Variation: WBVar00088906

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Name Class

WBVar00088906NamePublic_namemgDf90
Other_namemg90
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryGR
StatusLive
AffectsGeneWBGene00000899
Interactor (17)
DescriptionPhenotypeWBPhenotype:0000061Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Remark"When compared to the daf-2(e1370) parental strain, daf-2(e1370); daf-3(mgDf90) mutants lived significantly longer. This was also observed in daf-2(e1370); daf-3(e1376) worms, which is a weaker allele of daf-3." (see Figure 4A)Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Phenotype_assayGenotypedaf-2(e1370)Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
WBPhenotype:0000135Paper_evidenceWBPaper00054590
Curator_confirmedWBPerson712
Phenotype_assayGenotypedaf-3(mgDF90)X; muIs84[pAD76(psod-3::gfp)]; muEx541[pPC30(pgpa-4::GFP::Tetx) +punc-122::RFP]Paper_evidenceWBPaper00054590
Curator_confirmedWBPerson712
WBPhenotype:0000136Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Remark"We tested changes in the levels of these insulins using Q-PCR in TGFb pathway mutants such as daf-3(mgDf90), daf-14(m77) as well as pdp-1(tm3734) and compared them to wild-type worms (Figure 5A-5C, Figure S14, Tables S2 and S3). Interestingly, both pdp-1(tm3734) and daf-3(mgDf90) showed elevated levels of several insulins as compared to wild-type worms (Figure 5A and Figure S14). In contrast, expression of these insulins was markedly reduced in daf-14(m77) mutants (Figure 5B and Figure S14)."Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
daf-3(mgDf90) results in increased levels of pdp-1 mRNA, as determined by qRT-PCR (Figure S15)Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
WBPhenotype:0000637Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Remark"daf-2(e1370); daf-3(mgDf90) worms show significant enhancement of daf-2(e1370) dauer formation across several temperatures tested, whereas a daf-5 mutation or daf-5 RNAi results in reduced daf-2(e1370) dauer formation (Figure 4Ci, Figure 4Cii and Figure S11)."Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Phenotype_assayGenotypedaf-2(e1370)Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
WBPhenotype:0001090Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
RemarkFigure S13CPaper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
WBPhenotype:0001171Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
RemarkTable S1Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
WBPhenotype:0001655Paper_evidenceWBPaper00026674
Curator_confirmedWBPerson2987
RemarkTable 3Paper_evidenceWBPaper00026674
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00003022Paper_evidenceWBPaper00026674
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0001182Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Remark"We next tested the role of DAF-3 and DAF-5 on fat storage, dauer formation and stress resistance. Oil Red O staining for fat storage showed comparable levels between daf-2(e1370) and daf-2(e1370); daf-3(mgDf90) worms, but markedly lesser amounts of fat in daf-5(e1386); daf-2(e1370) worms (Figure 4B top and bottom panel and Figure S12)."Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Phenotype_assayGenotypedaf-2(e1370)Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
ReferenceWBPaper00038374
WBPaper00026674
WBPaper00011034
WBPaper00017169
WBPaper00054590
RemarkThis object is now known to be a deficiency and has been reassigned a Rearrangement object mgDf90Person_evidenceWBPerson545
Curator_confirmedWBPerson2970
MethodAllele