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WormBase Tree Display for Variation: WBVar00088704

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Name Class

WBVar00088704EvidencePaper_evidenceWBPaper00005086
NamePublic_namem540
Sequence_detailsSMapS_parentSequenceT13C5
Flanking_sequencesaagtttaaaattctaaaatattaaaatttcagatgaacataagccaaagttcttggataa
Mapping_targetT13C5
Type_of_mutationInsertion
SeqStatusSequenced
Variation_typeAllele
Transposon_insertion
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00006404
LaboratoryDR
StatusLive
AffectsGeneWBGene00000905
TranscriptT13C5.1b.1
T13C5.1a.1
InteractorWBInteraction000500281
WBInteraction000521459
WBInteraction000521462
WBInteraction000521465
GeneticsInterpolated_map_positionX-3.47349
DescriptionPhenotypeWBPhenotype:0000308Paper_evidenceWBPaper00005086
Curator_confirmedWBPerson712
RemarkAnimals resume development to fertile adults after 1-2 days. Recovery from Dauer and post recovery mophology is affected by cholesterol levels. Decreased cholesterol shifts the mutant phenotypes towards the stronger alleles; e.g. 20% recovery from Dauer, recovered adults are sick and sterile.Paper_evidenceWBPaper00005086
Curator_confirmedWBPerson712
WBPhenotype:0000447Paper_evidenceWBPaper00040979
Curator_confirmedWBPerson2987
Remark"Most daf-9(dh6) null animals developed into abnormal adults when supplemented with a minimum of 10 nM DA (Figure 2B, 74% +/- 42% non-dauers), suggesting that a threshold of DA has to be crossed before committing to adult fate (dauer bypass DA threshold). Increasing the levels to 25 nM DA decreased the frequency of dauers to 99% +/- 1% with about 66% of animals developing normally (Figure 2B). Further increase of DA to 50 nM increased the frequency of normal adults (Figure 2B). For a distribution of Mig and Cut phenotypes, see Figure S2. Similar results were observed with animals homozygous for daf- 9(e1406) or daf-9(m540) (Figure S2; worms were not synchronously hatched), both of which are strong loss-of-function alleles."Paper_evidenceWBPaper00040979
Curator_confirmedWBPerson2987
WBPhenotype:0001739Paper_evidenceWBPaper00045724
Curator_confirmedWBPerson4671
Remarkunable to respond to dietary restrictionPaper_evidenceWBPaper00045724
Curator_confirmedWBPerson4671
WBPhenotype:0001983Paper_evidenceWBPaper00032266
Curator_confirmedWBPerson712
RemarkMales homozygous for the hypomorphic allele daf-9(m540) had a slow rate of leaving like the daf-12 loss-of-function phenotype. Growth on DA-supplemented medium restored the leaving rate of adult males to the wild-type level.Paper_evidenceWBPaper00032266
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00032266
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000012Paper_evidenceWBPaper00005086
Curator_confirmedWBPerson712
WBPhenotype:0000061Paper_evidenceWBPaper00005086
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00005086
Curator_confirmedWBPerson712
Phenotype_assayTemperature15C, 20C, 25CPaper_evidenceWBPaper00005086
Curator_confirmedWBPerson712
WBPhenotype:0001653Paper_evidenceWBPaper00026674
Curator_confirmedWBPerson2987
RemarkTable 3Paper_evidenceWBPaper00026674
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00003022Paper_evidenceWBPaper00026674
Curator_confirmedWBPerson2987
ReferenceWBPaper00040979
WBPaper00032266
WBPaper00005086
WBPaper00026674
WBPaper00045724
MethodTransposon_insertion