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WormBase Tree Display for Variation: WBVar00088578

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Name Class

WBVar00088578EvidencePaper_evidenceWBPaper00015544
NamePublic_namem94
Other_nameCE01925:p.Glu781Lys
F30H5.1.1:c.2341G>A
HGVSgCHROMOSOME_III:g.499436G>A
Sequence_detailsSMapS_parentSequenceF30H5
Flanking_sequencestttgaaagagaaggcaattccaaagattgagaattctggtttatgacggatcacgagcattt
Mapping_targetF30H5
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00003295
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00006179
LaboratoryDR
StatusLive
AffectsGeneWBGene00006781
TranscriptF30H5.1.1 (12)
InteractorWBInteraction000050761
WBInteraction000050762
WBInteraction000534892
WBInteraction000534894
WBInteraction000534896
IsolationMutagenhydroxylamine
GeneticsInterpolated_map_positionIII-26.8216
DescriptionPhenotypeWBPhenotype:0000119Paper_evidenceWBPaper00065280
Curator_confirmedWBPerson712
RemarkWe induced UNC-45 dysfunction by RNAi-mediated depletion of unc-45 or by the unc-45(m94) ts mutant and observed stabilization of the UbV-mCherry protein specifically in BWM (Figure 1C and 1E). Since myosin is the major muscle protein, impairment of its folding by UNC-45 may lead to UPS overload in muscle.Paper_evidenceWBPaper00065280
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005813PATO:0000460Paper_evidenceWBPaper00065280
Curator_confirmedWBPerson712
WBPhenotype:0000273Paper_evidenceWBPaper00065280
Curator_confirmedWBPerson712
RemarkWhen grown at the permissive temperature of 15C, unc-45(m94) are superficially wild-type.Paper_evidenceWBPaper00065280
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00065280
Curator_confirmedWBPerson712
WBPhenotype:0000352Person_evidenceWBPerson2251
Curator_confirmedWBPerson2021
WBPhenotype:0000643Paper_evidenceWBPaper00046852
Person_evidenceWBPerson2251
Curator_confirmedWBPerson2021
WBPerson2987
Remark"A single amino acid substitute in the UCS domain of C-elegans UNC-45, E781K, or L822F (the m94 and e286 alleles, respectively), results in temperature-sensitive motility defects (Figure 1B) and myosin disorganization phenotypes (Figure 1C) when animals are grown at restrictive conditions (>22 degrees Celsius). However, animals show no phenotypes during development and early adulthood when grown at the permissive temperature (15 degrees Celsius C)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
WBPhenotype:0001403Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Remark"Animals expressing unc-45(e286) or unc-45(m94) that were shifted to restrictive conditions after the first larval stage exhibited disruption of myofilament structure associated with mislocalization of MYO-3, while wild-type animals were unaffected (Figure 3B)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Phenotype_assayTemperature25Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
WBPhenotype:0001569Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Remark"A single amino acid substitute in the UCS domain of C-elegans UNC-45, E781K, or L822F (the m94 and e286 alleles, respectively), results in temperature-sensitive motility defects (Figure 1B) and myosin disorganization phenotypes (Figure 1C) when animals are grown at restrictive conditions (>22 degrees Celsius). However, animals show no phenotypes during development and early adulthood when grown at the permissive temperature (15 degrees Celsius C)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
WBPhenotype:0001889Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Remark"Animals expressing unc-45(e286) or unc-45(m94) that were shifted to restrictive conditions after the first larval stage exhibited disruption of myofilament structure associated with mislocalization of MYO-3, while wild-type animals were unaffected (Figure 3B)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Phenotype_assayTemperature25Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000518Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Remark"A single amino acid substitute in the UCS domain of C-elegans UNC-45, E781K, or L822F (the m94 and e286 alleles, respectively), results in temperature-sensitive motility defects (Figure 1B) and myosin disorganization phenotypes (Figure 1C) when animals are grown at restrictive conditions (>22 degrees Celsius). However, animals show no phenotypes during development and early adulthood when grown at the permissive temperature (15 degrees Celsius C)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Phenotype_assayTemperature15Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
WBPhenotype:0000644Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson5063
RemarkAt 15C animals are WT on control RNAi but are fully paralyzed on daf-21, C01G10.8, sti-1 or ZC395.10 RNAi. WT animals do not show paralysis when treated with these RNAi. Similar phenotype can be observed when m94 is crossed to ok3501.Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson5063
Phenotype_assayTemperature15Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson5063
ReferenceWBPaper00015544
WBPaper00046852
WBPaper00065280
MethodSubstitution_allele