WormBase Tree Display for Variation: WBVar00060871
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WBVar00060871 | Name | Public_name | cxP9279 | |||||
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Variation_type | Allele | |||||||
Origin | Species | Caenorhabditis elegans | ||||||
Laboratory | LS | |||||||
Status | Dead | |||||||
Description | Phenotype | WBPhenotype:0000050 | Paper_evidence | WBPaper00049178 | ||||
Curator_confirmed | WBPerson712 | |||||||
Remark | The more significant effect of depleting cap-associated IFG-1 p170 was substantial embryonic lethality and arrest in early larval development. The majority of wild type eggs mature into L3 or L4 larvae by 48 h (Fig. 2H). | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00049178 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000054 | Paper_evidence | WBPaper00049178 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | The more significant effect of depleting cap-associated IFG-1 p170 was substantial embryonic lethality and arrest in early larval development. The majority of wild type eggs mature into L3 or L4 larvae by 48 h (Fig. 2H). By comparison, less than a third of ifg-1::mos eggs are able to enter larval development and these arrest at larval L1/L2 stages. By 96 h only 11 of those larvae (just under 7% of all eggs laid) reached maturity as adults. In comparison, over 191 wild type eggs (84%) developed into fertile adult offspring (Fig. 2I). | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00049178 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000120 | Paper_evidence | WBPaper00049178 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Properly spliced p130 and p170 mRNAs are translated into functional proteins. However, the overall protein abundance of both IFG-1 isoforms is diminished by 30% (Fig. 1D). Western blotting to detect both the p170 and p130 shows that loss of p170 protein expression (to 38% of wildtype levels) accounts for nearly all of the decrease. | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000121 | Paper_evidence | WBPaper00049178 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | A modest decrease in the translational efficiency of gpd-3 was detected in the ifg-1::mos strain indicated by the leftward shift in the mRNA distribution. gpd-3, however, still translated pretty efficiently on heavy polysomes (Fig. 3D). The ifg-1::mos showed consistent, modest increases in hsp-3 mRNA translational efficiency (Fig. 3F-G). This increase is significant due to the overall shift of the mRNA density into the more efficiently translating fractions near the bottom of the gradient. There was a slight decrease in the efficiency of hsp-4 translation in the ifg-1::mos strain (Fig. 3H) relative to wild type. ced-4 mRNA underwent a significant decrease in translational efficiency in the ifg-1::mos strain. | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00049178 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000154 | Paper_evidence | WBPaper00049178 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | We found only a modest decrease in the number of total eggs laid by the ifg-1::mos worms at 25 C (Fig. 2Hand 2I). The wild type strain laid about 228 eggs per hermaphrodite mother, whereas the ifg-1::mos strain laid about 148 eggs. | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00049178 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001180 | Paper_evidence | WBPaper00049178 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Small numbers of germ cell corpses begin to accumulate in both strainsas their gonads mature from the larval (L3) stage to the adult stage. This is followed by a steady and significant increase in apoptotic corpses (to an average of 13-15) in ifg-1::mos hermaphrodites in the young adult stages (24-48 h). By contrast, the steady-state number of apoptotic germ cells levels off at about 6h after wild type worms reach adulthood. The increase in apoptosis in the ifg-1::mos strain indicates that depleting IFG-1 p170 relative to p130 results in increased germ cell apoptosis. | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Genotype | CED-1::GFP | Paper_evidence | WBPaper00049178 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001807 | Paper_evidence | WBPaper00049178 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Mutants exhibit a 2.5-fold increase in the p130:p170 IFG-1 ratio (Fig. 1D). The change in isoform proportion is caused by a splicing defect in ifg-1 intron 5 that results from a Mos transposon insertion at an intron-exon junction(Fig. 1A). | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_not_observed | WBPhenotype:0000054 | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson1666 | |||||||
Remark | unlike ifg-1(ok1211) null mutant, larval development apparently normal; perhaps lengthened. | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson1666 | |||||||
WBPhenotype:0000062 | Paper_evidence | WBPaper00049178 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | a viable mutant worm strain | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000688 | Person_evidence | WBPerson1666 | ||||||
Curator_confirmed | WBPerson1666 | |||||||
Remark | unlike ifg-1(ok1211) null mutant, homozygous strain is reasonably fertile at 20C, slightly fertile at 25C | Person_evidence | WBPerson1666 | |||||
Curator_confirmed | WBPerson1666 | |||||||
WBPhenotype:0000691 | Paper_evidence | WBPaper00049178 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | strains showed normally developed gonads with a typical linear progression of developing germ cells that mature into oocytes | Paper_evidence | WBPaper00049178 | |||||
Curator_confirmed | WBPerson712 | |||||||
Reference | WBPaper00049178 | |||||||
Remark | [20051214 db] Renamed to cxTi9279 | |||||||
[Suppressed] This gene was previously annotaed as supressed. |