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WormBase Tree Display for Variation: WBVar00089700

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Name Class

WBVar00089700EvidencePaper_evidenceWBPaper00002413
Name (3)
Sequence_detailsSMapS_parentSequenceC48D1
Flanking_sequencestttttaaatgataattaataaatttttgcacaagtgtggagaaagaagccgagccaagct
Mapping_targetC48D1
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00002413
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00005600
WBStrain00005836
WBStrain00026860
WBStrain00026968
WBStrain00027213
WBStrain00027223
WBStrain00027232
WBStrain00027287
WBStrain00040671
WBStrain00040674
LaboratoryMT
StatusLive
AffectsGeneWBGene00000417
TranscriptC48D1.2a.1VEP_consequencesplice_acceptor_variant
VEP_impactHIGH
HGVScC48D1.2a.1:c.1210-1G>A
Intron_number7/9
C48D1.2b.1VEP_consequencesplice_acceptor_variant
VEP_impactHIGH
HGVScC48D1.2b.1:c.562-1G>A
Intron_number3/4
Interactor (29)
GeneticsInterpolated_map_positionIV8.46245
Mapping_dataIn_multi_point689
690
896
In_pos_neg_data831
DescriptionPhenotypeWBPhenotype:0000184Paper_evidenceWBPaper00044144
Curator_confirmedWBPerson2987
Remarkced-3(n717) completely suppressed the accumulation of germ cell corpses seen in dlc-1(RNAi) wormsPaper_evidenceWBPaper00044144
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeCED-1::GFP; dlc-1(RNAi)Paper_evidenceWBPaper00044144
Curator_confirmedWBPerson2987
WBPhenotype:0000216Paper_evidenceWBPaper00038432
Curator_confirmedWBPerson712
RemarkAnimals displayed a weak extra A/PVM phenotype.Paper_evidenceWBPaper00038432
Curator_confirmedWBPerson712
Phenotype_assayGenotypeayIs9[Pegl-17::gfp]Paper_evidenceWBPaper00038432
Curator_confirmedWBPerson712
WBPhenotype:0000386Paper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
RemarkCopper-induced germ cell apoptosis was abolished in ced-3(n717) mutants (Figure 2A).Paper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentWorms were exposed to 10 micromolar of copper for 12 hoursPaper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
WBPhenotype:0000414Paper_evidenceWBPaper00030973
Curator_confirmedWBPerson2021
RemarkThe sisters of ASEL and ASER (which normally undergo apoptosis) survive and adopt the same fate as ASEL and ASER respectivelyPaper_evidenceWBPaper00030973
Curator_confirmedWBPerson2021
PenetranceHigh95 percentPaper_evidenceWBPaper00030973
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0005952PATO:0000460Paper_evidenceWBPaper00030973
Curator_confirmedWBPerson2021
WBbt:0006657PATO:0000460Paper_evidenceWBPaper00030973
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentAll animals scored at 25C.Paper_evidenceWBPaper00030973
Curator_confirmedWBPerson2021
Temperature25CPaper_evidenceWBPaper00030973
Curator_confirmedWBPerson2021
GenotypeotIs114 [lim-6p::GFP], ntIs1 [gcy-5p::GFP]Paper_evidenceWBPaper00030973
Curator_confirmedWBPerson2021
WBPhenotype:0000590Paper_evidenceWBPaper00028561
Curator_confirmedWBPerson712
RemarkGermline cell corpse number is decreased compared to wild type.Paper_evidenceWBPaper00028561
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005784PATO:0000460Paper_evidenceWBPaper00028561
Curator_confirmedWBPerson712
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00028561
Curator_confirmedWBPerson712
Phenotype_assayTreatmentGermline corpses were quantified in staged adults (generally 24 hours after L4/adult molt) stained with SYTO12.Paper_evidenceWBPaper00028561
Curator_confirmedWBPerson712
Genotypeced-10(RNAi)Paper_evidenceWBPaper00028561
Curator_confirmedWBPerson712
WBPhenotype:0000965Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Remark12.11.1 (n=15) extra cells were counted in the anterior pharynx.Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Phenotype_assayTreatmentL3-L4 larvae were assayed.Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
WBPhenotype:0001014Paper_evidenceWBPaper00035076
Curator_confirmedWBPerson2021
RemarkSalmonella infection decreased the lifespan of wild-type worms more than that of ced-3(n717)-mutant animals, indicating that the ced-3 mutation protects against Salmonella infectionPaper_evidenceWBPaper00035076
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00035076
Curator_confirmedWBPerson2021
WBPhenotype:0001172Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkProgrammed cell deaths fail to occur. Very hard to score (ES1); difficult to score (ES2) in egl-1 background.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Ease_of_scoringES1_Very_hard_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001268Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
RemarkThe number of germ cell corpses per gonad arm was not changed above control levels after administration of 1uM ceramide.Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004926Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005175PATO:0000460Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Phenotype_assayTreatment1uM C16-ceramide was microinjected into the gonad.Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
WBPhenotype:0001270Paper_evidenceWBPaper00004574
Curator_confirmedWBPerson712
RemarkNo germ-line cell deaths were observed in a ced-3 mutant feeding on E. coli or S. typhimurium.Paper_evidenceWBPaper00004574
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005784PATO:0000460Paper_evidenceWBPaper00004574
Curator_confirmedWBPerson712
WBPhenotype:0001271Paper_evidenceWBPaper00004574
Curator_confirmedWBPerson712
Remarkced-3 mutants are hypersusceptible to S. typhimurium-mediated killing. ced-3 mutants died much more quickly than wild-type worms when feeding on S. typhimurium, but died at the same rate as wild-type worms when feeding on E. coli.Paper_evidenceWBPaper00004574
Curator_confirmedWBPerson712
WBPhenotype:0001620Paper_evidenceWBPaper00053621
Curator_confirmedWBPerson28450
WBPhenotype:0001621Paper_evidenceWBPaper00053621
Curator_confirmedWBPerson28450
WBPhenotype:0001964Paper_evidenceWBPaper00051461
Curator_confirmedWBPerson28450
WBPhenotype:0002541Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
RemarkRadiation-induced germ cell apoptosis was abolished.Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005784PATO:0000460Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAt least 20 animals were scored for germ cell apoptosis at 36 hours post-120 Gy and compared to WT-irradiated controls.Paper_evidenceWBPaper00032243
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000062Paper_evidenceWBPaper00038332
Curator_confirmedWBPerson712
RemarkAnimals produced viable progeny.Paper_evidenceWBPaper00038332
Curator_confirmedWBPerson712
WBPhenotype:0000072Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkNo gross phenotype.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000590Paper_evidenceWBPaper00038332
Curator_confirmedWBPerson712
WBPhenotype:0000966Paper_evidenceWBPaper00028759
Curator_confirmedWBPerson557
Remarkgermline is immortal at 20 and 25 degrees CelsiusPaper_evidenceWBPaper00028759
Curator_confirmedWBPerson557
Phenotype_assayTreatmentStrains propagated for many generations and checked for sterilityPaper_evidenceWBPaper00028759
Curator_confirmedWBPerson557
WBPhenotype:0001381Paper_evidenceWBPaper00046527
Curator_confirmedWBPerson2987
Remarkced-3(n717) mutant worms were not resistant to anoxia exposure when fed a glucose-supplemented diet (Figure S1C)Paper_evidenceWBPaper00046527
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00003916Paper_evidenceWBPaper00046527
Curator_confirmedWBPerson2987
WBPhenotype:0001650Paper_evidenceWBPaper00031571
Curator_confirmedWBPerson712
RemarkGerm cells are condensed and unhealthy although not as severely altered as those of at wild type or ced-9 animals.Paper_evidenceWBPaper00031571
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were grown on plates containing 400nM 5-FU for 12 hours from late L4 stage.Paper_evidenceWBPaper00031571
Curator_confirmedWBPerson712
WBPhenotype:0001651Paper_evidenceWBPaper00031571
Curator_confirmedWBPerson712
RemarkAnimals did not progress to L4 (data not shown).Paper_evidenceWBPaper00031571
Curator_confirmedWBPerson712
Phenotype_assayTreatmentEmbryos were transferred to plates containing 5-FU and remaining larva were counted after 72 hours.Paper_evidenceWBPaper00031571
Curator_confirmedWBPerson712
WBPhenotype:0001851Paper_evidenceWBPaper00027700
Curator_confirmedWBPerson2021
RemarkMutants did not display significant radiosensitivity when compared with WT N2 C. elegansPaper_evidenceWBPaper00027700
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0006748PATO:0000460Paper_evidenceWBPaper00027700
Curator_confirmedWBPerson2021
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00027700
Curator_confirmedWBPerson2021
Phenotype_assayTreatment100-400 Gy radiationPaper_evidenceWBPaper00027700
Curator_confirmedWBPerson2021
WBPhenotype:0002486Paper_evidenceWBPaper00050421
Curator_confirmedWBPerson2987
Remark"Mutations in genes within the ced-1/6/7 pathway (ced-1, ced-7, nrf-5, ttr-52), the ced-2/5/12 pathway (ced-2, ced-5), or both pathways (ced-1;ced-2 and ced-7;ced-5) did not cause PGC lobes to persist in L1 larvae (Supplementary Table 1), indicating that ced-10 functions in PGC lobe scission in a different context than it does in cell corpse engulfment." (PGC = 'primordial germ cell')Paper_evidenceWBPaper00050421
Curator_confirmedWBPerson2987
Variation_effectPredicted_null_via_sequencePaper_evidenceWBPaper00050421
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004576PATO:0000460Paper_evidenceWBPaper00050421
Curator_confirmedWBPerson2987
WBbt:0004575PATO:0000460Paper_evidenceWBPaper00050421
Curator_confirmedWBPerson2987
Life_stageWBls:0000024PATO:0000460Paper_evidenceWBPaper00050421
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentAll strains include the xnIs360 or xnSi1 transgenes to visualize PGC membranes.Paper_evidenceWBPaper00050421
Curator_confirmedWBPerson2987
Reference (33)
MethodSubstitution_allele