WormBase Tree Display for Transgene: WBTransgene00033745

WBTransgene00033745 Public_name: xchIs015
  Summary: [pLSD134 Phsp-16.2::ssSel1::FLAG::superfolderGFP::spacer::humanAmyloidBeta1-42::let-858-3UTR; pRF4 rol-6(su1006)].
  Construction: Construct: WBCnstr00004720
  Construction_summary: transgene that drives the expression via a heat shock promoter to induce and secret sfGFP::A1-42 (Jongsma et al., 2023).
  Genetic_information: Integrated
    Phenotype: WBPhenotype:0001405 Curator_confirmed: WBPerson712
        Remark: heat shock induced sfGFP::A1-42 expression and secretion with 3 hours of heat shock (Figure 1A). "we assessed sfGFP::A1-42 aggregation levels after 48 hours post-induction. Fluorescent images of the control group revealed extensive induction and aggregation of A in the cuticle, coelomocytes, and the tail (Figure 1E)."; "Screening results of various drugs indicated optimal dosages and combinations, with quercetin 350 M and rifampicin 75 M producing the largest visible reduction in amyloid burden. Higher dosages of drugs resulted in extensive death of C. elegans with rifampicin 175-200 M, quercetin 450-500 M, and alpha-ketoglutarate (aKG) 12-16 mM (Figure 1C, Screen 1)." Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00001874 Curator_confirmed: WBPerson712
            WBMol:00003989 Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Curator_confirmed: WBPerson712
  Reference: WBPaper00066270
  Species: Caenorhabditis elegans