WormBase Tree Display for Transgene: WBTransgene00021303
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WBTransgene00021303 | Public_name | ltSi794 | |||||
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Summary | [pOD1988/pSW302; Pdpy-7::vhhGFP4::ZIF-1::unc-54_3'UTR; cb-unc-119(+)] | ||||||
Construction | Construct | WBCnstr00020930 | |||||
Integration_method | Single_copy_insertion | ||||||
Genetic_information | Integrated | ||||||
Map | II | ||||||
Phenotype | WBPhenotype:0000036 | Paper_evidence | WBPaper00048540 | ||||
Curator_confirmed | WBPerson12586 | ||||||
Remark | Surviving worms homozygous for lt1, lt19 and ltSi794 are about half the size of age-matching wild type. | Paper_evidence | WBPaper00048540 | ||||
Curator_confirmed | WBPerson12586 | ||||||
WBPhenotype:0000060 | Paper_evidence | WBPaper00048540 | |||||
Curator_confirmed | WBPerson12586 | ||||||
Remark | 80% of worms homozygous for lt1, lt19 and ltSi794 rupture and die at 72-hour after bleach synchronization and release. | Paper_evidence | WBPaper00048540 | ||||
Curator_confirmed | WBPerson12586 | ||||||
WBPhenotype:0000685 | Paper_evidence | WBPaper00048540 | |||||
Curator_confirmed | WBPerson12586 | ||||||
Remark | lt19 is a GFP knock-in at the C-terminal of gip-2 locus, while ltSi794 transgene encodes a GFP nanobody::ZIF-1 fusion specifically in the epidermis. This fusion protein degrades GIP-2::GFP. Worms homozygous for lt1, lt19 and ltSi794 grow slower than controls during larval development. | Paper_evidence | WBPaper00048540 | ||||
Curator_confirmed | WBPerson12586 | ||||||
Associated_with | Strain | WBStrain00029236 | |||||
Reference | WBPaper00048540 | ||||||
WBPaper00051381 | |||||||
Species | Caenorhabditis elegans |