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WormBase Tree Display for Transgene: WBTransgene00021303

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Name Class

WBTransgene00021303Public_nameltSi794
Summary[pOD1988/pSW302; Pdpy-7::vhhGFP4::ZIF-1::unc-54_3'UTR; cb-unc-119(+)]
ConstructionConstructWBCnstr00020930
Integration_methodSingle_copy_insertion
Genetic_informationIntegrated
MapII
PhenotypeWBPhenotype:0000036Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
RemarkSurviving worms homozygous for lt1, lt19 and ltSi794 are about half the size of age-matching wild type.Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
WBPhenotype:0000060Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
Remark80% of worms homozygous for lt1, lt19 and ltSi794 rupture and die at 72-hour after bleach synchronization and release.Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
WBPhenotype:0000685Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
Remarklt19 is a GFP knock-in at the C-terminal of gip-2 locus, while ltSi794 transgene encodes a GFP nanobody::ZIF-1 fusion specifically in the epidermis. This fusion protein degrades GIP-2::GFP. Worms homozygous for lt1, lt19 and ltSi794 grow slower than controls during larval development.Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
Associated_withStrainWBStrain00029236
ReferenceWBPaper00048540
WBPaper00051381
SpeciesCaenorhabditis elegans