WormBase Tree Display for Transgene: WBTransgene00021165
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| WBTransgene00021165 | Public_name | qxIs495 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Summary | [Pced-1::PPK-1::GFP] | ||||||||
| Construction | Construct | WBCnstr00020776 | |||||||
| Genetic_information | Integrated | ||||||||
| Phenotype | WBPhenotype:0001181 | Paper_evidence | WBPaper00048406 | ||||||
| Curator_confirmed | WBPerson17560 | ||||||||
| Remark | Figure 1H, over expression of PPK-1 causes accumulated somatic cell corpses. "Overexpression of PPK-1 or inactivation of ocrl-1 affected apoptotic cell clearance, causing accumulation of cell corpses, indicating the importance of maintaining PtdIns(4,5)P2 and MTM-1 dynamics in this process (Fig 1 H; Fig 2, P-X; and Fig S4 G)." | Paper_evidence | WBPaper00048406 | ||||||
| Curator_confirmed | WBPerson17560 | ||||||||
| Caused_by_gene | WBGene00004087 | Paper_evidence | WBPaper00048406 | ||||||
| Curator_confirmed | WBPerson17560 | ||||||||
| WBPhenotype:0001846 | Paper_evidence | WBPaper00048406 | |||||||
| Curator_confirmed | WBPerson17560 | ||||||||
| Remark | "PPK-1 expression significantly extended phagosomal duration of PLCδ1-PH, suggesting that PPK-1 produces PtdIns(4,5)P2 on forming phagosomes (Fig 1, E and G). Similarly, MTM-1(C378S) appeared simultaneously with PPK-1 and persisted for significantly longer on phagosomes when PPK-1 was coexpressed (Fig 1, F and G)... Overexpression of PPK-1 extended the PtdIns(4,5)P2-positive stage, causing prolonged phagosomal association of MTM-1(C378S) (Fig 1, E-G)... the appearance of 2xFYVE on phagosomes was severely impaired, suggesting that sustained MTM-1 suppresses PtdIns3P accumulation (Fig 4, B, D, and G)... Overexpression of PPK-1 or inactivation of OCRL-1, which causes increased PtdIns(4,5)P2 on phagosomes, led to extended LST-4 duration accompanied by defective 2xFYVE appearance (Fig 2, V-X; Fig 6 C; and Fig S4, B-F)." | Paper_evidence | WBPaper00048406 | ||||||
| Curator_confirmed | WBPerson17560 | ||||||||
| Caused_by_gene | WBGene00004087 | Paper_evidence | WBPaper00048406 | ||||||
| Curator_confirmed | WBPerson17560 | ||||||||
| EQ_annotations | Molecule_affected | WBMol:00001619 | PATO:0000460 | Paper_evidence | WBPaper00048406 | ||||
| Curator_confirmed | WBPerson17560 | ||||||||
| Reference | WBPaper00048406 | ||||||||
| Species | Caenorhabditis elegans |
