WormBase Tree Display for Transgene: WBTransgene00021094
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| WBTransgene00021094 | Public_name | otIs334 | |
|---|---|---|---|
| Summary | [sox-3 fosmid::mCherry, pRF4 rol-6(su1006)] | ||
| Construction | Construct | WBCnstr00020693 | |
| Integration_method | Gamma_irradiation | ||
| Construction_summary | Recombineered fosmids were linearized with SdaI and injected at 15 ng/ul into N2 worms, together with ScaI-digested rol-6(d) (pRF4; 5 ng/ul) and PvuII-digested bacterial genomic DNA (100 ng/ul) to generate a complex array. Integrated transgenes were generated bygamma-irradiation and outcrossed four to six times. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr12602 | |
| Associated_with | Strain | WBStrain00029608 | |
| Reference | WBPaper00047042 | ||
| Species | Caenorhabditis elegans |
