WormBase Tree Display for Transgene: WBTransgene00020208
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| WBTransgene00020208 | Public_name | krSi3 | |
|---|---|---|---|
| Summary | [Punc-36::egfp::unc-36, Cbr-unc-119 (+)] | ||
| Synonym | UNC-36-GFP | ||
| Construction | Construct | WBCnstr00019650 | |
| Integration_method | Single_copy_insertion | ||
| Construction_summary | We followed the direct-insertion MosSCI protocol (Frkjaer-Jensen et al., 2008) to generate krSi3. In brief, we micro-injected the gonads of EG4322 worms with a DNA mix containing: the targeting vector pHZ025 at 50 ng/uL, pJL43.1 at 50 ng/uL (Mos transposases expressed under germline constitutive promoter Pglh-2), and transformation markers Pmyo3::gfp at 10 ng/uL and Prab3::gfp at 10 ng/uL. MosSCI events were selected by screening for the restoration of wild-type locomotion and loss of the transformation markers. The krSi3 insertion was validated by PCR using oCF418 5'-TCTGGCTCTGCTTCTTCGTT-3' and oCF419 5'-CAA TTCA TCCCGGTTTCTGT-3' (Frkjaer-Jensen et al., 2008). | ||
| Genetic_information | Integrated | ||
| Map | II | ||
| Used_for | Expr_pattern | Expr11978 | |
| Expr11979 | |||
| Associated_with | Strain | WBStrain00042968 | |
| Reference | WBPaper00045731 | ||
| WBPaper00045754 | |||
| Species | Caenorhabditis elegans |
