WormBase Tree Display for Transgene: WBTransgene00020143
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| WBTransgene00020143 | Public_name | xnIs459 | |
|---|---|---|---|
| Summary | [Pral-1::yfp-ral-1a] | ||
| Construction | Construct | WBCnstr00019548 | |
| Integration_method | Particle_bombardment | ||
| Construction_summary | Plasmids were mixed 1:1 with pMA122 (Frkjr-Jensen et al., 2012), which carries a heat-shock inducible peel-1 toxin. Transformed, non-Unc worms were propagated several generations, and then heat-shocked at 34 deg C for 2 h to induce expression of the toxin, selecting against worms inheriting multi-copy transgenes (those containing pMA122). At least two independent lines were analyzed for each construct. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr11911 | |
| Associated_with | Strain | WBStrain00007543 | |
| Reference | WBPaper00045599 | ||
| WBPaper00053041 | |||
| WBPaper00053837 | |||
| Species | Caenorhabditis elegans |
