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WormBase Tree Display for Transgene: WBTransgene00019307

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Name Class

WBTransgene00019307Public_nameqnIs142
Summary[Phsp-16.2:nlp-22; Phsp-16.2:gfp; Pmyo-2:mCherry, unc-119(+)]
ConstructionConstructWBCnstr00018645
WBCnstr00000570
Coinjection_otherPmyo-2:mCherry, unc-119(+)
Genetic_informationIntegrated
PhenotypeWBPhenotype:0000019Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
RemarkAlthough kin-2 mutants continued to pump upon nlp-22 overexpression, pumping rates were reduced relative to kin-2-mutant controls (Supplementary Table S1)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Heat-shock-induced overexpression of NLP-22 resulted in reduced pharyngeal pumping rates in the egl-4(n479) mutant background (Table S1)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003760Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Phenotype_assayGenotypekin-2(ce179)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
egl-4(n479)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
WBPhenotype:0000353Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
RemarkUnder mild heat-shock conditions, in which nlp-22-overexpressing adult animals were not completely quiescent, they moved backwards more often than control animals (Fig. 3b) .Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003760Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
WBPhenotype:0000523Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
RemarkSimilar to wild-type worms in lethargus, adult worms overexpressing nlp-22 showed reduced responsiveness to optic (blue light) or chemical (1-octanol) stimulation compared with transgenic control animals that overexpressed GFP (Fig. 3a)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003760Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
WBPhenotype:0001524Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
RemarkWhen authors overexpressed nlp-22 using an inducible heatshock promoter (Fig. 2a) during the normally active adult period, they observed quiescence of feeding and locomotion (Fig. 2b, c, Supplementary Movie 1, Supplementary Table S1). Two hours after heat shock, control animals made 15.9 +/- 7.3 (all values presented as mean +/- s.d.) (N=15) anterior body bends per min, whereas animals overexpressing nlp-22 made 0.55 +/- 1.1 (N=25) body bends per min (Fig. 2b). Wild-type animals and transgenic control animals expressing GFP under the control of a heat-shock promoter pumped 2 h after heat shock at rates of 264.9 +/- 17.4 (N=20) and 273.6 +/- 17.5 (N=20) pumps per min, respectively (Fig. 2c, Supplementary Table S1). Animals carrying the integrated or extrachromosomal Phsp16.2:nlp-22 arrays, qnIs142 or qnEx95, pumped at rates of 18.6 +/- 31.1 (N=37) and 25.2 +/- 32.3 (N=40) per min, respectively, 2 h after heat shock (Fig. 2c, Supplementary Table S1).Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003760Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
WBPhenotype:0001897Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
RemarkSimilar to wild-type worms in lethargus, adult worms overexpressing nlp-22 showed reduced responsiveness to optic (blue light) or chemical (1-octanol) stimulation compared with transgenic control animals that overexpressed GFP (Fig. 3a)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003760Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Associated_withStrainWBStrain00044205
ReferenceWBPaper00044546
WBPaper00048737
WBPaper00059841
SpeciesCaenorhabditis elegans