WormBase Tree Display for Transgene: WBTransgene00019307
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WBTransgene00019307 | Public_name | qnIs142 | ||||||
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Summary | [Phsp-16.2:nlp-22; Phsp-16.2:gfp; Pmyo-2:mCherry, unc-119(+)] | |||||||
Construction | Construct | WBCnstr00018645 | ||||||
WBCnstr00000570 | ||||||||
Coinjection_other | Pmyo-2:mCherry, unc-119(+) | |||||||
Genetic_information | Integrated | |||||||
Phenotype | WBPhenotype:0000019 | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Although kin-2 mutants continued to pump upon nlp-22 overexpression, pumping rates were reduced relative to kin-2-mutant controls (Supplementary Table S1) | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Heat-shock-induced overexpression of NLP-22 resulted in reduced pharyngeal pumping rates in the egl-4(n479) mutant background (Table S1) | Paper_evidence | WBPaper00044546 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003760 | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | kin-2(ce179) | Paper_evidence | WBPaper00044546 | ||||
Curator_confirmed | WBPerson2987 | |||||||
egl-4(n479) | Paper_evidence | WBPaper00044546 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000353 | Paper_evidence | WBPaper00044546 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Under mild heat-shock conditions, in which nlp-22-overexpressing adult animals were not completely quiescent, they moved backwards more often than control animals (Fig. 3b) . | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003760 | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000523 | Paper_evidence | WBPaper00044546 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Similar to wild-type worms in lethargus, adult worms overexpressing nlp-22 showed reduced responsiveness to optic (blue light) or chemical (1-octanol) stimulation compared with transgenic control animals that overexpressed GFP (Fig. 3a) | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003760 | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001524 | Paper_evidence | WBPaper00044546 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | When authors overexpressed nlp-22 using an inducible heatshock promoter (Fig. 2a) during the normally active adult period, they observed quiescence of feeding and locomotion (Fig. 2b, c, Supplementary Movie 1, Supplementary Table S1). Two hours after heat shock, control animals made 15.9 +/- 7.3 (all values presented as mean +/- s.d.) (N=15) anterior body bends per min, whereas animals overexpressing nlp-22 made 0.55 +/- 1.1 (N=25) body bends per min (Fig. 2b). Wild-type animals and transgenic control animals expressing GFP under the control of a heat-shock promoter pumped 2 h after heat shock at rates of 264.9 +/- 17.4 (N=20) and 273.6 +/- 17.5 (N=20) pumps per min, respectively (Fig. 2c, Supplementary Table S1). Animals carrying the integrated or extrachromosomal Phsp16.2:nlp-22 arrays, qnIs142 or qnEx95, pumped at rates of 18.6 +/- 31.1 (N=37) and 25.2 +/- 32.3 (N=40) per min, respectively, 2 h after heat shock (Fig. 2c, Supplementary Table S1). | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003760 | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001897 | Paper_evidence | WBPaper00044546 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Similar to wild-type worms in lethargus, adult worms overexpressing nlp-22 showed reduced responsiveness to optic (blue light) or chemical (1-octanol) stimulation compared with transgenic control animals that overexpressed GFP (Fig. 3a) | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003760 | Paper_evidence | WBPaper00044546 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Associated_with | Strain | WBStrain00044205 | ||||||
Reference | WBPaper00044546 | |||||||
WBPaper00048737 | ||||||||
WBPaper00059841 | ||||||||
Species | Caenorhabditis elegans |