WormBase Tree Display for Transgene: WBTransgene00018085
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WBTransgene00018085 | Public_name | WBPaper00041849Ex1 | |
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Summary | [GLB-26::GFP] | ||
Synonym | Expr10701_Ex | ||
Construction | Construct | WBCnstr00017475 | |
Construction_summary | The translational reporter for glb-26 was constructed using fusion PCR, as described by Hobert and coworkers. The reporter contains 2.12 kb upstream and 0.90 kb downstream of the glb-26 gene, to include endogenous promoter and 3'UTR elements. The gfp gene was amplified from the vector pPD95.75 (Fire Lab), and fused at the 3' side of the glb-26 coding gene, thereby preceding the glb-26 3'UTR region. For glb-26, the primers used were the forward primer 5'-TGAAGATGGTGGTACAAAGT-3' to amplify the promoter sequence, the forward nested primer 5'-GTAAAACTTTGGGTTGGTCT-3' for the promoter sequence, the reverse primer 5'-AGTTCTTCTCCTTTACTCAACTCCTCATCGTCTTCTTTTG-3' for the glb-26 gene, the glb-26 3'UTR forward primer 5'- GCATGGATGAACTATACAAATGAATGTGTGATTTTTTGAT-3', the glb-26 3'UTR reverse primer 5'-GAAATGTGCTCTCTATGAGG- 3', and the glb-26 3'UTR reverse nested primer 5'-GCACTTGTGACGTTTTCTAT-3'. For the gfp gene, the forward primer 5'-TTGAGTAAAGGAGAAGAAC-3' and the reverse primer 5'- TTTGTATAGTTCATCCATGCC-3' were used. | ||
Genetic_information | Extrachromosomal | ||
Used_for | Expr_pattern | Expr10701 | |
Reference | WBPaper00041849 | ||
Species | Caenorhabditis elegans |