WormBase Tree Display for Transgene: WBTransgene00018036
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| WBTransgene00018036 | Public_name | WBPaper00041646Ex1 | |
|---|---|---|---|
| Summary | [Pfaah-1::gfp] | ||
| Synonym | Expr10655_Ex | ||
| Construction | Construct | WBCnstr00017426 | |
| Construction_summary | To make Pfaah-1::gfp, a 3-kb genomic DNA of the faah-1 promoter region in N2 was amplified by PCR with the following primers:F1: 5'-GCATGCgaatcttcatcctctgatggctgcat-3'; R1: 5'-GGTACCgatgaccttgaaatactgaaaattgaaagtg-3'.The primers introduced an SphI (forward primer) and a KpnI (reverse primer) endonuclease recognition sites. Using the SphI and KpnI sites, the amplified faah-1 promoter DNA was inserted into the pPD95.75 vector. --Precise ends. | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr10655 | |
| Reference | WBPaper00041646 | ||
| Species | Caenorhabditis elegans |
