WormBase Tree Display for Transgene: WBTransgene00015971
expand all nodes | collapse all nodes | view schema
| WBTransgene00015971 | Public_name | drSi13 | |
|---|---|---|---|
| Summary | [hsf-1p::hsf-1::GFP::unc-54utr; Cbr-unc-119(+)] | ||
| Synonym | [HSF-1::GFP] | ||
| Construction | Construct | WBCnstr00015540 | |
| Coinjection_other | Cbr-unc-119(+) | ||
| Integration_method | Single_copy_insertion | ||
| Construction_summary | Four kb of genomic sequence upstream of the hsf-1 start ATG (inclusive) was PCR amplified from C. elegans N2 genomic DNA with added attB sites and cloned into the Gateway promoter vector pDONRP4-P1R (primers OG371 and OG937; plasmid pOG88). C. elegans hsf-1 cDNA was cloned from EST clone yk609a8 into Gateway entry clone pDONR221, including the start and stop codons (primers OG289, OG290, followed by PCR with attB adaptor primers OG78 and OG79; plasmid pOG37). The first base pair of the start codon and last two of the stop codon were deleted by site-directed mutagenesis (QuikChange II Kit, Cat. #200523) in order to put the clone in frame for Gateway (primers OG396, OG400, OG475, OG476; plasmid pOG34). GFP with the 3' UTR of unc-54 was cloned from pPD95.75 (which contains synthetic introns) into Gateway cloning vector pDONRP2R-P3 (OG949, OG950; plasmid pOG99). | ||
| Genetic_information | Integrated | ||
| Map | II | ||
| Used_for | Expr_pattern | Expr10595 | |
| Interactor | WBInteraction000519256 | ||
| Associated_with | Strain | WBStrain00029262 | |
| Reference (15) | |||
| Species | Caenorhabditis elegans |
