WormBase Tree Display for Transgene: WBTransgene00015954
expand all nodes | collapse all nodes | view schema
WBTransgene00015954 | Public_name | smIs98 | |||||
---|---|---|---|---|---|---|---|
Summary | [Pmec-3::GFP; Pmec-7::HBx] | ||||||
Construction | Construct | WBCnstr00015523 | |||||
Genetic_information | Integrated | ||||||
Map | II | ||||||
Phenotype | WBPhenotype:0000183 | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | ||||||
Remark | "To better analyze the cell-killing activity of HBx, we expressed HBx in six mechanosensory neurons under the control of the mec-7 gene promoter (Pmec-7HBx) (Fig. S1A). To aid in identification of these nonessential neurons, we expressed GFP under the control of the mec-3 promoter (Pmec-3GFP), which drives gene expression in the same six touch cells plus four other sensory neurons (Fig. S1A). An integrated transgene (smIs98) containing both Pmec-7HBx and Pmec-3GFP was used to assay killing of touch cells by HBx (Fig. S1A). On average, 13-50% of touch cells in smIs98 animals underwent ectopic cell death, with the two posterior lateral microtubule (PLM) neurons showing most ectopic deaths (50%; Fig. S1B)." | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | ||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | ||||||
WBPhenotype:0001397 | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | ||||||
Remark | "To better analyze the cell-killing activity of HBx, we expressed HBx in six mechanosensory neurons under the control of the mec-7 gene promoter (Pmec-7HBx) (Fig. S1A). To aid in identification of these nonessential neurons, we expressed GFP under the control of the mec-3 promoter (Pmec-3GFP), which drives gene expression in the same six touch cells plus four other sensory neurons (Fig. S1A). An integrated transgene (smIs98) containing both Pmec-7HBx and Pmec-3GFP was used to assay killing of touch cells by HBx (Fig. S1A). On average, 13-50% of touch cells in smIs98 animals underwent ectopic cell death, with the two posterior lateral microtubule (PLM) neurons showing most ectopic deaths (50%; Fig. S1B)." | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | ||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | ||||||
WBPhenotype:0002615 | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | ||||||
Remark | "Some dying touch cells in smIs98 animals displayed an enlarged vacuolar morphology characteristic of necrotic cells (Fig. 1D) and some displayed the raised disk-like morphology of apoptotic cells (Fig. 1E)." | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | ||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | ||||||
Reference | WBPaper00041673 | ||||||
Species | Caenorhabditis elegans |