Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Transgene: WBTransgene00015954

expand all nodes | collapse all nodes | view schema

Name Class

WBTransgene00015954Public_namesmIs98
Summary[Pmec-3::GFP; Pmec-7::HBx]
ConstructionConstructWBCnstr00015523
Genetic_informationIntegrated
MapII
PhenotypeWBPhenotype:0000183Paper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
Remark"To better analyze the cell-killing activity of HBx, we expressed HBx in six mechanosensory neurons under the control of the mec-7 gene promoter (Pmec-7HBx) (Fig. S1A). To aid in identification of these nonessential neurons, we expressed GFP under the control of the mec-3 promoter (Pmec-3GFP), which drives gene expression in the same six touch cells plus four other sensory neurons (Fig. S1A). An integrated transgene (smIs98) containing both Pmec-7HBx and Pmec-3GFP was used to assay killing of touch cells by HBx (Fig. S1A). On average, 13-50% of touch cells in smIs98 animals underwent ectopic cell death, with the two posterior lateral microtubule (PLM) neurons showing most ectopic deaths (50%; Fig. S1B)."Paper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
Caused_by_otherhepatitis B virus protein HBxPaper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
WBPhenotype:0001397Paper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
Remark"To better analyze the cell-killing activity of HBx, we expressed HBx in six mechanosensory neurons under the control of the mec-7 gene promoter (Pmec-7HBx) (Fig. S1A). To aid in identification of these nonessential neurons, we expressed GFP under the control of the mec-3 promoter (Pmec-3GFP), which drives gene expression in the same six touch cells plus four other sensory neurons (Fig. S1A). An integrated transgene (smIs98) containing both Pmec-7HBx and Pmec-3GFP was used to assay killing of touch cells by HBx (Fig. S1A). On average, 13-50% of touch cells in smIs98 animals underwent ectopic cell death, with the two posterior lateral microtubule (PLM) neurons showing most ectopic deaths (50%; Fig. S1B)."Paper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
Caused_by_otherhepatitis B virus protein HBxPaper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
WBPhenotype:0002615Paper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
Remark"Some dying touch cells in smIs98 animals displayed an enlarged vacuolar morphology characteristic of necrotic cells (Fig. 1D) and some displayed the raised disk-like morphology of apoptotic cells (Fig. 1E)."Paper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
Caused_by_otherhepatitis B virus protein HBxPaper_evidenceWBPaper00041673
Curator_confirmedWBPerson2987
ReferenceWBPaper00041673
SpeciesCaenorhabditis elegans