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WormBase Tree Display for Transgene: WBTransgene00015425

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Name Class

WBTransgene00015425Public_nameWBPaper00040999Ex1
Summary[ifo-1::yfp and ifo-1::cfp]
SynonymExpr10040_Ex
ConstructionConstructWBCnstr00015016
Construction_summaryConstructs encoding IFO-1::YFP and IFO-1::CFP were prepared by amplifying the 1256 bp ifo-1 promoter from genomic DNA (amplimers: 5'- CCGCCGAAGCTTTGGTTTTAAATTGTATTTTATAG-3', 5'-CCGC- CGAAGCTTGCTGAAATCGTATTCGAATTTTG-3') and inserting the HindIII-cleaved fragment into the yfp- and cfp-containing vectors pVH20.01 and pVH10.10 (Hutter, 2003) generating pPifo-1::yfp and pPifo-1::cfp, respectively. For cloning of the 3897 bp ifo-1 cDNA fragment, total RNA was prepared (RNeasy Minikit, Qiagen) and reverse transcribed (Omniscript RT kit, Qiagen). ifo-1 cDNA was then amplified by PCR (primers: 5'-ATCATCGGATCCATGGGAGACCTACAAGTCGAC-3', 5'- TCCTCCGGATCCAAATTTGGTCCATCGCCGG-3'). The BamHI- digested product was inserted into pPifo-1::yfp and pPifo-1::cfp.
Genetic_informationExtrachromosomal
Used_forExpr_patternExpr10040
ReferenceWBPaper00040999
SpeciesCaenorhabditis elegans