WormBase Tree Display for Transgene: WBTransgene00009217
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| WBTransgene00009217 | Public_name | rmEx260 | |||||
|---|---|---|---|---|---|---|---|
| Summary | [pF25B3.3::257cAT3v1-1q128::yfp] | ||||||
| Synonym | 275cAT3q128 | ||||||
| Construction | Construct | WBCnstr00008904 | |||||
| Integration_method | Gamma_irradiation | ||||||
| Construction_summary | ATXN3 C-terminal YFP-tagged expression constructs (lacking 257aa from the amino terminal) were generated by PCR (using full-length constructs as a template) and cloned into the BamH I sites of PF25B3.3Q0::YFP, forming PF25B3.3::257cAT3q(n)::YFP. | ||||||
| Genetic_information | Extrachromosomal | ||||||
| Phenotype | WBPhenotype:0000229 | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Animals were smaller than animals expressing full-length AT3q130 (ataxin with polyQ(130) expansion) or WT controls. | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_other | ATXN3 lacking amino terminal 257 aa, with 128 polyQ | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0000306 | Paper_evidence | WBPaper00038396 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | This ATXN3 C-terminal YFP-tagged expression construct containing a 128 polyQ tract (275cAT3q75) forms immobile aggregates in all neurons tested by FRAP. | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_other | ATXN3 lacking amino terminal 257 aa, with polyQ(75) expansion | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0000646 | Paper_evidence | WBPaper00038396 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Animals were more lethargic than WT controls and animals expressing a ATXN3 poyQ(130) expansion. | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_other | ATXN3 lacking amino terminal 257 aa, with 128 polyQ | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0000679 | Paper_evidence | WBPaper00038396 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Unlike in animals expressing full-length AT3q14 (ataxin with polyQ(14) expansion) or YFP-alone, discrete foci formed throughout different cells of the nervous system. | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_other | ATXN3 lacking amino terminal 257 aa, with 128 polyQ | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0000848 | Paper_evidence | WBPaper00038396 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Animals developed to adulthood one day later than control animals. | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_other | ATXN3 lacking amino terminal 257 aa, with 128 polyQ | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Phenotype_not_observed | WBPhenotype:0000688 | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Animals had progeny. | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_other | ATXN3 lacking amino terminal 257 aa, with 128 polyQ | Paper_evidence | WBPaper00038396 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Reference | WBPaper00038396 | ||||||
| Species | Caenorhabditis elegans |
