WormBase Tree Display for Transgene: WBTransgene00009113
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WBTransgene00009113 | Public_name | kcEx19 | ||||||
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Summary | [sng-1p::sng-1; ifb-2p::yfp] | |||||||
Construction | Construct | WBCnstr00008801 | ||||||
Construction_summary | For preparation of rescue construct sng-1p::sng-1, a 553 bp XmaI/NcoI-flanked fragment was amplified with primers 06-117 (5-CAT CCC GGG TAG TTT TTT AGC CAA AC-3) and 06-118 (5-CCA CCA TGG GTT GAA AAT AAA ACT TGA AG-3) from cosmid T08A9 and cloned into the sng-1::yfp-containing vector. The transcriptional reporter construct ifb-2p::yfp was prepared by amplifying the 1994 bp ifb-2 promoter fragment from genomic DNA as described (Husken et al., 2008) and inserting it into the HindII/PstI sites of the yfp-containing vector pVH20.01 (Hutter, 2003). | |||||||
Genetic_information | Extrachromosomal | |||||||
Phenotype | WBPhenotype:0000010 | Paper_evidence | WBPaper00039786 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Animals showed slightly increased PTZ sensitivity at 4 mg/ml and a significant increase in PTZ sensitivity was noted at 8 mg/ml in comparison to the wild-type. At 8 mg/ml PTZ the number of cramping animals was similar to that observed for sng-1 mutants. | Paper_evidence | WBPaper00039786 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00004912 | Paper_evidence | WBPaper00039786 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00004251 | Paper_evidence | WBPaper00039786 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000017 | Paper_evidence | WBPaper00039786 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Worms overexpressing synaptogyrin (strain BJ120) were more ric than sng-1(ok234) single mutants. | Paper_evidence | WBPaper00039786 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00004912 | Paper_evidence | WBPaper00039786 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00003650 | Paper_evidence | WBPaper00039786 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0004022 | Paper_evidence | WBPaper00039786 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Animals showed highly significant differences in the amplitude of the sinusoidal motility of wild-type. | Paper_evidence | WBPaper00039786 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00004912 | Paper_evidence | WBPaper00039786 | |||||
Curator_confirmed | WBPerson712 | |||||||
Reference | WBPaper00039786 | |||||||
Species | Caenorhabditis elegans |