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WormBase Tree Display for Transgene: WBTransgene00009113

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Name Class

WBTransgene00009113Public_namekcEx19
Summary[sng-1p::sng-1; ifb-2p::yfp]
ConstructionConstructWBCnstr00008801
Construction_summaryFor preparation of rescue construct sng-1p::sng-1, a 553 bp XmaI/NcoI-flanked fragment was amplified with primers 06-117 (5-CAT CCC GGG TAG TTT TTT AGC CAA AC-3) and 06-118 (5-CCA CCA TGG GTT GAA AAT AAA ACT TGA AG-3) from cosmid T08A9 and cloned into the sng-1::yfp-containing vector. The transcriptional reporter construct ifb-2p::yfp was prepared by amplifying the 1994 bp ifb-2 promoter fragment from genomic DNA as described (Husken et al., 2008) and inserting it into the HindII/PstI sites of the yfp-containing vector pVH20.01 (Hutter, 2003).
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000010Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
RemarkAnimals showed slightly increased PTZ sensitivity at 4 mg/ml and a significant increase in PTZ sensitivity was noted at 8 mg/ml in comparison to the wild-type. At 8 mg/ml PTZ the number of cramping animals was similar to that observed for sng-1 mutants.Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
Caused_by_geneWBGene00004912Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004251Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
WBPhenotype:0000017Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
RemarkWorms overexpressing synaptogyrin (strain BJ120) were more ric than sng-1(ok234) single mutants.Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
Caused_by_geneWBGene00004912Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003650Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
WBPhenotype:0004022Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
RemarkAnimals showed highly significant differences in the amplitude of the sinusoidal motility of wild-type.Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
Caused_by_geneWBGene00004912Paper_evidenceWBPaper00039786
Curator_confirmedWBPerson712
ReferenceWBPaper00039786
SpeciesCaenorhabditis elegans