WormBase Tree Display for Transgene: WBTransgene00009023
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| WBTransgene00009023 | Public_name | syIs218 | |
|---|---|---|---|
| Summary | [Pgcy-8::chR2::yfp; Ppax-2::gfp; lin-15(+)] | ||
| Construction | Construct | WBCnstr00008712 | |
| Coinjection_other | pHC294.1 [Ppax-2::gfp], lin-15(+) | ||
| Integration_method | X-ray | ||
| Construction_summary | This transgene was constructed by injecting gcy-8::chR2::yfp plasmid DNA into MT1642 lin-15(n765ts) worms with the coinjection marker pHC294.1, a pax-2::gfp plasmid with GFP expression in the vulva and tail. PCR was used to amplify the 2.258-kb fragment upstream of the gcy-8 gene using the primers 5'-TCCCCCGGGATCTTGAGGACCTCGTCTTTAAGG-3' and 5'-CGCGGATCCTTTGATGTGGAAAAGGTAGAATCGAAAATCC-3', and cloned into the Pml1 and BamH1 sites of the Pmyo-3::ChR2(H134R)::YFP plasmid to make gcy-8::chR2::yfp. | ||
| Genetic_information | Integrated | ||
| Reference | WBPaper00038440 | ||
| Species | Caenorhabditis elegans |
