WormBase Tree Display for Transgene: WBTransgene00006461
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| WBTransgene00006461 | Public_name | csEx72 | |
|---|---|---|---|
| Summary | [hsp::lin-45ED + sur-5::GFP] | ||
| Synonym | (hsp16-41-lin-45ED) | ||
| [Phsp16.41::lin-45ED, sur-5::GFP] | |||
| Construction | Construct | WBCnstr00006308 | |
| Construction_summary | LIN-45 ED replaces activating phosphorylation sites Thr 626 and Thr 629 with glutamic acid and aspartic acid, respectively, to mimic constitutive phosphorylation. hsp1641-lin-45ED plasmid pCR33.1 was generated by cloning a PCR product amplified from plasmid lin-45ED (32) with primers CRo181 (5'-TTC ATG GCT AGC ACC ATG AGT CGG ATT AAT TTC AAA AAG TC-3') and CRo182 (5'-TTC ATG CCA TGG CTA AAT GAG ACC ATA GAC ATT G-3') into the NheI and NcoI sites of heat-shock vector pPD49.83. pCR33.1 was injected into N2 animals at 20 ng/l with pTG_96 (sur-5::gfp) (33) at 30 ng/l and pBluescript at 50 ng/l to yield transgenic strain csEx72. | ||
| Genetic_information | Extrachromosomal | ||
| Associated_with | Strain | WBStrain00035419 | |
| Reference | WBPaper00026726 | ||
| WBPaper00031642 | |||
| Species | Caenorhabditis elegans |
