WormBase Tree Display for Transgene: WBTransgene00005543

WBTransgene00005543 Public_name: wuIs152
  Summary: [sod-1 (genomic) + pRF4 (rol-6)]
  Synonym: [sod-1(+)]
  Construction: Construct: WBCnstr00005461
    Coinjection: WBCnstr00004720
    Integration_method: X-ray
  Genetic_information: Integrated
    Phenotype: WBPhenotype:0000061 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: Animals display an long-life span compared to control animals. This extended life span was not suppressed by NAC, a broad-spectrum antioxidant. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003315 Paper_evidence: WBPaper00040082
              Curator_confirmed: WBPerson712
      WBPhenotype:0001236 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: sod-1 OE increased expression of hsp-4::gfp and hsp-6::gfp, a marker of the mitochondrial UPR. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
      WBPhenotype:0001644 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: Overexpression of SOD-1 causes a 1.5-fold increase in protein damage as measured by an increase in protein carbonyl levels. Levels of oxidized proteins is elevated. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
      WBPhenotype:0001719 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: sod-1 OE increased expression of hsp-4::gfp and hsp-6::gfp, a marker of the mitochondrial UPR. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
      WBPhenotype:0002003 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: Animals show a modest but statistically significant increase in steady-state H2O2 levels. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000725 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: Overexpression of SOD-1 does not affect levels of 4-hydroxynonenal (HNE) protein adduct, a measure of lipid oxidation. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
      WBPhenotype:0001273 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: No difference was observed between sod-1 OE and wild-type worms in resistance to heat stress (35 C). Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
      WBPhenotype:0001349 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: sod-1 OE did not detectably increase AAK-2 phosphorylation levels. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
      WBPhenotype:0002008 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: Overexpression of SOD-1 does not affect levels of carboxymethllysine (CML), a measure of glycation activity. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
      WBPhenotype:0002009 Paper_evidence: WBPaper00040082
        Curator_confirmed: WBPerson712
        Remark: Overexpression of SOD-1 does not affect the turnover rate for oxidized proteins that are processed through the proteasome core complex although an increase in oxidized proteins is observed. Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004930 Paper_evidence: WBPaper00040082
            Curator_confirmed: WBPerson712
  Used_for: Interactor: WBInteraction000500819
      WBInteraction000500820
      WBInteraction000500821
      WBInteraction000500822
      WBInteraction000517296
  Associated_with: Strain: WBStrain00007673
      WBStrain00007674
      WBStrain00007678
  Reference: WBPaper00032404
    WBPaper00032989
    WBPaper00035663
    WBPaper00040082
    WBPaper00041882
    WBPaper00045770
  Species: Caenorhabditis elegans