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WormBase Tree Display for Transgene: WBTransgene00005249

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Name Class

WBTransgene00005249Public_namezxIs6
Summary[unc-17p::ChR2(H134R)::YFP + lin-15(+)]
Synonym[Punc-17::chop-2(H134R)-yfp; lin-15(+)]
[punc-17::ChR2(H134R)::YFP; lin-15+]
ConstructionConstructWBCnstr00005226
Genetic_informationIntegrated
PhenotypeWBPhenotype:0000153Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
RemarkAnimals showed rapid and sustained body contraction to 92% of intial body length after illumination (photoactivation of ChR2-YFP in cholinergic neurons).Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Caused_by_otherHuman channelrhodopsin-2 (H134R)Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_assayTreatmentChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
WBPhenotype:0000567Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
RemarkDorsal coiling was triggered within ~10 sec of photostimulation of ChR2-YFP in cholinergic neurons.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Caused_by_otherHuman channelrhodopsin-2 (H134R)Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_assayTreatmentChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0001316Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
RemarkExogenous neurotransmitter evoked currents were no different from wild-type.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Caused_by_otherHuman channelrhodopsin-2 (H134R)Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_assayTreatmentChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
WBPhenotype:0001318Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
RemarkThe frequency and amplitude of endogenous miniature postsynaptic currents of photostimulated ChR2-YFP containing cholinergic neurons were comparable to wild-type.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Caused_by_otherHuman channelrhodopsin-2 (H134R)Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_assayTreatmentChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Associated_withStrainWBStrain00040913
WBStrain00055728
WBStrain00055731
Reference (39)
SpeciesCaenorhabditis elegans