WormBase Tree Display for Transgene: WBTransgene00004854
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| WBTransgene00004854 | Public_name | urIs261 | |
|---|---|---|---|
| Summary | [unc-6::unc-6(+)(IM#184); pRF4] | ||
| Construction | Construct | WBCnstr00004830 | |
| Coinjection | WBCnstr00004720 | ||
| Integration_method | Gamma_irradiation | ||
| Construction_summary | IM#97 is the plasmid construct of unc-6 genomic sequence. The plasmid pIM#180 was constructed by deleting the eight amino acids-like region in domain VI. To make the deletion, two fragments that introduce a HinfI site were PCR-amplified by using pIM#97 as a template. The primer pair for the first fragment was 5 -GTAGCACCTAGGGTG-3 and 5 -CTTGTAAAGTGCCATTGAATCTGGGAGA-3 . After the PCR, the PCR-amplified products were digested with SalI and HinfI. The primer sets for the second fragment were 5 -TCTCCCAGATTCAATGGCACTTTACAAGACCCCGTTTCAATTCTACTCCTCCGAATGTCG-3 and 5 -GTCAGTTGCGGTGACCCAATCC-3 . For this PCR, the same template was used, and the amplified PCR products were digested with HinfI and SgrAI. A 906 bp fragment was excised with SalI and SgrAI from pIM#97 and replaced by the above SalIHinfI and HinfISgrAI fragments to generate pIM#180. --precise ends. | ||
| Genetic_information | Integrated | ||
| Associated_with | Strain | WBStrain00022090 | |
| WBStrain00022093 | |||
| WBStrain00022096 | |||
| Reference | WBPaper00005417 | ||
| Species | Caenorhabditis elegans |
