WormBase Tree Display for Transgene: WBTransgene00001256
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| WBTransgene00001256 | Public_name | ncIs30 | |
|---|---|---|---|
| Summary | [plx-2::gfp] | ||
| Construction | Construct | WBCnstr00001247 | |
| Integration_method | Gamma_irradiation | ||
| Construction_summary | Two translational fusion constructs were made. A full length PLX-2::GFP translational fusion construct (KKB75) was made by subcloning genomic fragments of K04B12 into pPD95.75, except for the 3' terminal fragment (3320 to 2675) which was amplified and cloned into the Kpn I site of pPD95.75. KKB75 was coinjected (0.2 ug/l) with pRF4 to generate extrachromosomal arrays that were integrated by gamma-ray mutagenesis to create ncIs30. --precise ends.|For the partial translational fusion construct, a PCR fragment corresponding to the plx-2 genomic DNA, 20517 to 6426 nt of K04B12, was cloned into BamH I-Sal I digested pFXneEGFP, resulting in the translational fusion of the N-terminal half of PLX-2 (residues 1 to 888) with EGFP (Living Colors Fluorescent Proteins, Clontech). | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr4682 | |
| Reference | WBPaper00029396 | ||
| Species | Caenorhabditis elegans |
