WormBase Tree Display for Transgene: WBTransgene00000933
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WBTransgene00000933 | Public_name | maIs100 | |
---|---|---|---|
Summary | [daf-7(+); lin-4(+)] | ||
Construction | Construct | WBCnstr00000927 | |
Integration_method | Gamma_irradiation | ||
Construction_summary | The 693-bp lin-4-rescuing fragment was amplified with PCR primers RFMGH25 and RFMGH46. The lin-4 DNA PCR product (200 ng/ul) and approximately 10 ng/ul of cosmid DE9 [daf-7(+)] were injected into daf-7(e1372ts) animals raised at 15C using standard microinjection procedures. One transgenic extrachromosomal array that conferred a high penetrance of the precocious phenotypes was integrated by gamma-ray irradiation. The two resulting independent integrated transgenic lines were VT731 daf-7(e1372ts); maIs101[daf-7(+);lin-4(+)] and VT780 daf-7(e1372ts); maIs100[daf-7(+);lin-4(+)]. | ||
Genetic_information | Integrated | ||
Used_for | Interactor | WBInteraction000501674 | |
Reference | WBPaper00003583 | ||
Species | Caenorhabditis elegans |