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WormBase Tree Display for Transgene: WBTransgene00000933

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Name Class

WBTransgene00000933Public_namemaIs100
Summary[daf-7(+); lin-4(+)]
ConstructionConstructWBCnstr00000927
Integration_methodGamma_irradiation
Construction_summaryThe 693-bp lin-4-rescuing fragment was amplified with PCR primers RFMGH25 and RFMGH46. The lin-4 DNA PCR product (200 ng/ul) and approximately 10 ng/ul of cosmid DE9 [daf-7(+)] were injected into daf-7(e1372ts) animals raised at 15C using standard microinjection procedures. One transgenic extrachromosomal array that conferred a high penetrance of the precocious phenotypes was integrated by gamma-ray irradiation. The two resulting independent integrated transgenic lines were VT731 daf-7(e1372ts); maIs101[daf-7(+);lin-4(+)] and VT780 daf-7(e1372ts); maIs100[daf-7(+);lin-4(+)].
Genetic_informationIntegrated
Used_forInteractorWBInteraction000501674
ReferenceWBPaper00003583
SpeciesCaenorhabditis elegans