WormBase Tree Display for Strain: WBStrain00055400
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| WBStrain00055400 | Genotype | hint-1(sy1567) I; hint-3(sy2026) V. | ||
|---|---|---|---|---|
| Public_name | PS9997 | |||
| Contains | Gene | WBGene00009002 | ||
| WBGene00016150 | ||||
| Variation | WBVar02159047 | |||
| WBVar02159137 | ||||
| Properties | Outcrossed | x0 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 02 Aug 2023 00:00:00 | |||
| Location | CGC | |||
| Remark | Superficially wild type. Penetrance lethal with lots of unhatched eggs and slow growth. CRISPR/Cas9 engineered STOP-IN null mutant of hint-3 into hint-1(sy1567) stop-in mutant. Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). Left flanking sequence: catgttttacaggATGACGTCAATGCATACTTCTGT. Right flanking sequence: TAACGGATGCAAGTTTTGTGACATTGTCAAAAATA. Inserted sequence between the two-flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TCAATGCATACTTCTGTTAA. Method Reference: Wang H, et al. G3 (Bethesda). 2018 Nov 6;8(11):3607-3616. | Inferred_automatically | From CGC strain data | |
| Made_by: Heenam Park | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
