WormBase Tree Display for Strain: WBStrain00055270
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| WBStrain00055270 | Genotype | lgc-22(sy1478) IV. | ||
|---|---|---|---|---|
| Public_name | PS8708 | |||
| Contains | Gene | WBGene00017483 | ||
| Variation | WBVar02159045 | |||
| Properties | Outcrossed | x0 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 15 Jun 2022 00:00:00 | |||
| Location | CGC | |||
| Remark | Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of lgc-22. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: GGCTTCTCATTGCCACGTCACTGGCCGCCACGTTA right flanking sequence: GCGTGGGCGGCGGGCACCGCCGGCGGCTGCGAGGAC inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: CACTGGCCGCCACGTTAGCG Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616 | Inferred_automatically | From CGC strain data | |
| Made_by: Heenam Park & Mandy Tan | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
