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WormBase Tree Display for Strain: WBStrain00054678

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Name Class

WBStrain00054678Genotypecat-2(cer181[cat-2p::gfp::H2B 1-3]) II.
Public_nameCER588
ContainsGeneWBGene00000296
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received29 Jul 2022 00:00:00
LocationCGC
RemarkAbnormal locomotion can be rescued with dopamine. cat-2(cer181) is a complete deletion of the cat-2 gene (coding sequence + introns), which was substituted by the sequence of the step 1 repair for GFP::H2B (Nested CRISPR, Vicencio et al, Genetics 2019). Allele can be detected using the following primers: Fwd: ctatgtgaagtcacacctgtc Rev: cttgctggaagtgtacttggtg. Reference: Martnez-Fernndez C, et al. Dis Model Mech. 2022 Mar 1;15(3):dmm049161. doi: 10.1242/dmm.049161. PMID: 35107130Inferred_automaticallyFrom CGC strain data
Made_by: Carmen Martnez-FernndezCGC_data_submission
SpeciesCaenorhabditis elegans