WormBase Tree Display for Strain: WBStrain00037463

WBStrain00037463 Status: Live
  Genotype: rpm-1(ju1928) degt-1(ok3307) V.
  Public_name: VC2633
  Contains: Gene: WBGene00004457
      WBGene00009109
    Variation: WBVar02125402
      WBVar02159024
  Properties: Outcrossed: x1
    Mutagen: EMS
    CGC_received: 07 Mar 2014 00:00:00
    Phenotype: WBPhenotype:0002069 Paper_evidence: WBPaper00064137
        Curator_confirmed: WBPerson557
        Remark: Animals showed a defect in the PLM neurons axon termination (overextension). Paper_evidence: WBPaper00064137
            Curator_confirmed: WBPerson557
  Location: CGC
    VC
  Made_by: WBPerson20176
  Remark: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence: WBPaper00041807
    F25D1.4. External left primer: TCAAGGAAGCATCCGAAGTT. External right primer: CCACGGATGAATCGAGTTTT. Internal left primer: TCAGATTTTTGGAGTTTCCGA. Internal right primer: TTATTCGATTTTCCCCGTTG. Internal WT amplicon: 1152 bp. Deletion size: 915 bp. Deletion left flank: TTTTGGAGTTTCCGATAATTTCCATGATGT. Deletion right flank: TTCAGTGATAAATTTTCAAATTTCTCGAAA. [NOTE: (05/10/2022) This strain also carries an (A to T) missense mutation in rpm-1 which results in a Q3089H amino acid substitution in RPM-1. See Jin EJ & Jin Y. (2022). A mutation linked to degt-1(ok3307) in C. elegans strain VC2633 affects rpm-1. microPublication Biology. 10.17912/micropub.biology.000565. PMC ID: PMC9073554.] Inferred_automatically: From CGC strain data
  Reference: WBPaper00059258
    WBPaper00065804
    WBPaper00064137
  Species: Caenorhabditis elegans