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WormBase Tree Display for Strain: WBStrain00037115

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Name Class

WBStrain00037115StatusLive
GenotypeC06B8.7(ok2814) V/nT1 [qIs51] (IV;V).
Public_nameVC2150
ContainsGeneWBGene00007372
VariationWBVar00093911
RearrangementnT1
nT1[qIs51]
TransgeneWBTransgene00001903
PropertiesOutcrossedx1
MutagenEMS
CGC_received26 Jun 2008 00:00:00
LocationCGC
Made_byWBPerson20180
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
C06B8.7. Homozygous viable deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2814 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TCACAGAGCGATGGTACTCG. External right primer: CCACCTCGAACCGTTTTCTA. Internal left primer: TGCAGATTCAAACCCATCAA. Internal right primer: TCCAACATTCCTTGCGTGTA. Internal WT amplicon: 1163 bp. Deletion size: 680 bp. Deletion left flank: GCTTAAAGCAGGAGAGACCAAAGCGTGCTT. Deletion right flank: TCTCCGAATGATCGTATCACACTGGCCAAC.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans