WormBase Tree Display for RNAi: WBRNAi00107832

WBRNAi00107832 Homol: Homol_homol: T23G11:RNAi
  Sequence_info: PCR_product: sjj_T23G11.3
  Experiment: Genotype: GFP::LGG-2
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: T23G11.3 Inferred_automatically: RNAi_primary
    Gene: WBGene00001595 Inferred_automatically: RNAi_primary
    Transcript: T23G11.3.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Interaction: WBInteraction000535527
  Reference: WBPaper00049105
  Phenotype: WBPhenotype:0001276 Remark: "Since many tumors require drastic changes in metabolism and particularly in catabolism to grow, we asked whether autophagy is induced in germline tumors in C. elegans. To test this, we examined whether autophagy-related proteins are expressed in the wild-type gonad and/or in the gld-1 germline tumor. We used transgenic animals that express fluorescence tagged versions of the following proteins: GFP::LGG-1 (GABARAP ortholog), GFP::LGG-2 (LC3 ortholog), and BEC-1::GFP (BECN1 ortholog). Consistent with previous reports, we observed that autophagy-related proteins are highly expressed in the nervous system, pharynx, intestine, hypodermis, vulva, spermatheca and somatic gonad of wild-type animals, but not in wild-type germline cells (Fig 1B). However, cells within the germline tumor in gld-1 RNAi animals show high expression of the autophagy pathway proteins from the first day of adulthood throughout their life span (Fig 1C and D). Notably, these transgenes (with the exception of BEC-1::GFP) are not expressed in wild-type germlines and only become activated during zygotic gene activation in embryos (Fig 1B) or somatic trans-differentiation in the tumor."
      EQ_annotations: Anatomy_term: WBbt:0005784 PATO:0000460
        GO_term: GO:0006914 PATO:0000460
  Remark: (Figure 1B-D) gld-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
  Method: RNAi