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WormBase Tree Display for RNAi: WBRNAi00103798

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Name Class

WBRNAi00103798HomolHomol_homolT24D8:RNAi
Sequence_infoDNA_textaacaattaacatcagaaaattcatactccgattgggaatccagttagttcgtccgttgttcgtttccctggacggaatccggctcgcccaatcgcaatcgactttttcatgattggaagggttcggagtctccaatagcgcaagcgcccctcacgagtgttgcgcaggtagtttgctcggtgaaggatctgattgaaatcgacttgatcgacaactcctaatccgcggaagacatcgattccaccctgaaattacggtgcttgttgccatttgaaggaactcgattttgggaatttttgaacttatactttcaaaatatcctcaaatgaatgaattttttaataaactttcgcgaaagtttttcaatatttttttgtggcggttcaaaattttcgcataaaaatggcctaattcggacggaaattgaaattttgtaacctattggttgcctatccgcttgaaattttaaacttaaaaaaacgatgtaaatgtgcaaattgagtcaataataaaaaaaaaaaatttttggggatttaaggctaatttacaaacaaacacatctaggtcttttttcgaaaatttgaaccgtcgtaaacacaatttgatcggtacccttttcctttaacattgtgtaaagctaa
ExperimentLaboratoryNQ
Date30 Oct 2013 00:00:00
Genotypesid-1(pk3321) V; qnIs157 [glr-3:nlp-22(RNAi); myo-2:mCherry; unc-119(+)]
Delivered_byTransgene_expression
InhibitsPredicted_geneT24D8.3Inferred_automaticallyRNAi_primary
GeneWBGene00003760Inferred_automaticallyRNAi_primary
TranscriptT24D8.3.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00044546
PhenotypeWBPhenotype:0001524RemarkAuthors expressed nlp-22 double-stranded RNA (dsRNA) to knockdown nlp-22 mRNA specifically in RIA neurons, and, as a control, in the hypodermis. Expression in the RIA neurons but not in the hypodermis reduced lethargus quiescence (Fig. 4b,c and Supplementary Table S2).
Remark(Figure 4b, c, Table S2) RIA-neuron-specific nlp-22 RNAi. Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.
MethodRNAi