WormBase Tree Display for RNAi: WBRNAi00102855
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| WBRNAi00102855 | Homol | Homol_homol | C44B7:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_C44B7.9 | |||
| Experiment | Laboratory | KQ | |||
| Date | 06 Dec 2013 00:00:00 | ||||
| Treatment | Synchronized L1 animals were transferred to plates containing RNAi/bacteria. They were fed on RNAi plates throughout their growth and treatment. The identity of each RNAi clone was blinded during experiments. Knockdowns that resulted in a suppression of the dopamine induced fat phenotype of more than 80% of the control animal's reduction were considered for further analyses. For the metabolic screen, data were acquired with Acumen (TTP LABTECH, England) a microplate fluorescence citometer, and double checked in a visual-based assay. For missing values or values with SD bigger than 20% from the automatic assay, only the visual-based assay data were considered. Data from both assays had the same trend with the former being more sensitive for variations in intensity. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | C44B7.9 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00004059 | Inferred_automatically | RNAi_primary | ||
| Transcript | C44B7.9.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000525119 | ||||
| Reference | WBPaper00044757 | ||||
| Phenotype | WBPhenotype:0000523 | Remark | pmp-2 RNAi worms exhibit an attenuated response (little or no reduction in fat content, as determined by Nile Red staining) to exogenous dopamine (5mM), compared to controls | ||
| Affected_by | Molecule | WBMol:00004583 | |||
| Remark | (Figure S7) pmp-2/C44B7.9 RNAi | ||||
| Method | RNAi |
