WormBase Tree Display for RNAi: WBRNAi00102347
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WBRNAi00102347 | Homol | Homol_homol | D2024:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | mv_D2024.7 | |||
Experiment | Laboratory | RD | |||
Date | 07 Jul 2009 00:00:00 | ||||
Strain | WBStrain00000001 | ||||
Treatment | RNAi strain cultures were grown for 6 hours in LB medium containing 100 mg/mL ampicillin, and then spread onto NGM agar containing isopropyl thio-beta-D-galactoside (1 mM) and carbenicillin (25 micrograms per millilter). For double- RNAi treatment, equal concentrations of both strains were mixed before seeding. The next day, animals at the fourth larval stage were placed onto RNAi plates, grown for 2 days and then harvested by rinsing with M9 buffer (0.1 M NaCl, 0.05 M potassium phosphate, pH 6.0). Adults were allowed to settle, and eggs were recovered from hermaphrodites by alkaline hypochlorite lysis (5 min at room temperature in 0.5 M NaOH, 5% hypochlorite). The eggs were rinsed with M9 buffer and the resulting L1 larvae were transferred the next day to fresh agar plates containing the different dsRNA conditions. | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | D2024.7 | Inferred_automatically | RNAi_primary | |
Gene | WBGene00017054 | Inferred_automatically | RNAi_primary | ||
Transcript | D2024.7.1 | Inferred_automatically | RNAi_primary | ||
Species | Caenorhabditis elegans | ||||
Interaction | WBInteraction000524868 | ||||
Reference | WBPaper00035091 | ||||
Phenotype_not_observed | WBPhenotype:0000138 | ||||
Remark | (Figure 6) gstk-2 RNAi | ||||
Method | RNAi |