WormBase Tree Display for RNAi: WBRNAi00101780

WBRNAi00101780 Homol: Homol_homol: C10G8:RNAi
  Sequence_info: PCR_product: mv_C10G8.6
  Experiment: Laboratory: LW
    Date: 04 May 2009 00:00:00
    Genotype: jjIs1475(myo-3::rfp) I; rrf-3(pk1426) II; ccIs4438 (intrinsic CC(CoelomoCyte)::gfp) III; ayIs2(egl-15::gfp) IV; ayIs6(hlh-8::gfp) X
    Treatment: To observe the effects of RNAi during postembryonic development, RNAi was performed by ingestion with a synchronous population of L1 larvae. L1 animals synchronized by standard methods were plated in triplicate on HT115(DE3) bacteria expressing dsRNA for genes of interest. Bacteria for ingestion were prepared using 4 mM IPTG to induce dsRNA production. RNAi-P was performed at 25 degrees Celsius and animals were scored for M lineage phenotypes or used for immunostaining 24-48 h after plating.
    Temperature: 25
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: C10G8.6 Inferred_automatically: RNAi_primary
    Gene: WBGene00000455 Inferred_automatically: RNAi_primary
    Transcript: C10G8.6.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00033137
  Phenotype: WBPhenotype:0000095 Remark: Postembryonic RNAi targeting ceh-34 resulted in adult animals lacking the two posterior (M-lineage derived) coelomocytes (CCs), as indicated by loss of expression of the 'intrinsic CC::gfp' coelomocyte marker in the posterior of the adult animal. The M lineage was followed in these animals using the hlh-8::gfp reporter and anti-FOZI-1 immunostaining, and normal cleavage orientations and proliferation were observed in the M lineage. However, at the 18-M stage, both M.drpa and M.dlpa were transformed from CCs to body wall muscles (BWMs; as observed using myo-3::rfp) in 10 of 11 animals examined, while the remaining animal had only one CC transformed to a BWM (data not shown).
    WBPhenotype:0000414 Remark: Postembryonic RNAi targeting ceh-34 resulted in adult animals lacking the two posterior (M-lineage derived) coelomocytes (CCs), as indicated by loss of expression of the 'intrinsic CC::gfp' coelomocyte marker in the posterior of the adult animal. The M lineage was followed in these animals using the hlh-8::gfp reporter and anti-FOZI-1 immunostaining, and normal cleavage orientations and proliferation were observed in the M lineage. However, at the 18-M stage, both M.drpa and M.dlpa were transformed from CCs to body wall muscles (BWMs; as observed using myo-3::rfp) in 10 of 11 animals examined, while the remaining animal had only one CC transformed to a BWM (data not shown).
    WBPhenotype:0000852 Remark: Postembryonic RNAi targeting ceh-34 resulted in adult animals lacking the two posterior (M-lineage derived) coelomocytes (CCs), as indicated by loss of expression of the 'intrinsic CC::gfp' coelomocyte marker in the posterior of the adult animal. The M lineage was followed in these animals using the hlh-8::gfp reporter and anti-FOZI-1 immunostaining, and normal cleavage orientations and proliferation were observed in the M lineage. However, at the 18-M stage, both M.drpa and M.dlpa were transformed from CCs to body wall muscles (BWMs; as observed using myo-3::rfp) in 10 of 11 animals examined, while the remaining animal had only one CC transformed to a BWM (data not shown).
    WBPhenotype:0000961 Remark: Postembryonic RNAi targeting ceh-34 resulted in adult animals lacking the two posterior (M-lineage derived) coelomocytes (CCs), as indicated by loss of expression of the 'intrinsic CC::gfp' coelomocyte marker in the posterior of the adult animal. The M lineage was followed in these animals using the hlh-8::gfp reporter and anti-FOZI-1 immunostaining, and normal cleavage orientations and proliferation were observed in the M lineage. However, at the 18-M stage, both M.drpa and M.dlpa were transformed from CCs to body wall muscles (BWMs; as observed using myo-3::rfp) in 10 of 11 animals examined, while the remaining animal had only one CC transformed to a BWM (data not shown).
    WBPhenotype:0001912 Remark: Postembryonic RNAi targeting ceh-34 resulted in adult animals lacking the two posterior (M-lineage derived) coelomocytes (CCs), as indicated by loss of expression of the 'intrinsic CC::gfp' coelomocyte marker in the posterior of the adult animal. The M lineage was followed in these animals using the hlh-8::gfp reporter and anti-FOZI-1 immunostaining, and normal cleavage orientations and proliferation were observed in the M lineage. However, at the 18-M stage, both M.drpa and M.dlpa were transformed from CCs to body wall muscles (BWMs; as observed using myo-3::rfp) in 10 of 11 animals examined, while the remaining animal had only one CC transformed to a BWM (data not shown).
  Phenotype_not_observed: WBPhenotype:0000669 Remark: Fate specification of sex myoblasts (SMs; visualized using hlh-8::gfp) and their derivatives (visualized using egl-15::gfp) was unaffected in ceh-34 (RNAi-P) animals.
  Remark: (Figure 1B,D, Table 1) ceh-34 RNAi-P (postembryonic RNAi)
  Method: RNAi