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WormBase Tree Display for RNAi: WBRNAi00101622

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Name Class

WBRNAi00101622HomolHomol_homolW06F12:RNAi
Sequence_infoPCR_productsjj_W06F12.1
ExperimentLaboratoryCT
Date01 Nov 2010 00:00:00
GenotypewIs78 [SCMp::GFP marker transgene for seam cells]; lin-42(n1089)
TreatmentRNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneW06F12.1aInferred_automaticallyRNAi_primary
W06F12.1dInferred_automaticallyRNAi_primary
W06F12.1eInferred_automaticallyRNAi_primary
W06F12.1cInferred_automaticallyRNAi_primary
W06F12.1bInferred_automaticallyRNAi_primary
GeneWBGene00003048Inferred_automaticallyRNAi_primary
TranscriptW06F12.1a.1Inferred_automaticallyRNAi_primary
W06F12.1c.1Inferred_automaticallyRNAi_primary
W06F12.1b.1Inferred_automaticallyRNAi_primary
W06F12.1e.1Inferred_automaticallyRNAi_primary
W06F12.1d.2Inferred_automaticallyRNAi_primary
W06F12.1d.1Inferred_automaticallyRNAi_primary
W06F12.1d.3Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00037838
PhenotypeWBPhenotype:0000168Remarklit-1 RNAi partially suppressed the precocious production of alae in L4 larvae animals carrying the lin-42(n1089) mutation
Remark(Figure 7C) lit-1 RNAi
MethodRNAi