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WormBase Tree Display for RNAi: WBRNAi00101300

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Name Class

WBRNAi00101300HomolHomol_homolT06E6:RNAi
Sequence_infoPCR_productsjj_T06E6.2
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); POPTOP (POP-1 and TCF Optimal Promoter) consisting of (7x POP-1 binding sites)::(pes-10 minimal promoter)::mCherry [as a Wnt/Beta-catenin pathway activity reporter]
TreatmentSynchronized L1s of strains carrying POPTOP; rrf-3(pk1426) were fed on RNAi bacteria for 24 hours at 22 degrees Celsius. Early-to-mid L3 male animals were scored for mCherry positive cells in the gonad.
Delivered_byBacterial_feeding
InhibitsPredicted_geneT06E6.2aInferred_automaticallyRNAi_primary
T06E6.2bInferred_automaticallyRNAi_primary
GeneWBGene00000868Inferred_automaticallyRNAi_primary
TranscriptT06E6.2b.1Inferred_automaticallyRNAi_primary
T06E6.2a.1Inferred_automaticallyRNAi_primary
T06E6.2a.2Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0001279Remarkcyb-3 RNAi resulted in reduced numbers of POPTOP mCherry-positive cells in the male gonad, compared to control
EQ_annotationsLife_stageWBls:0000035PATO:0000460
Remark(Figure 5B) cyb-3 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi