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WormBase Tree Display for RNAi: WBRNAi00099346

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Name Class

WBRNAi00099346HomolHomol_homolCHROMOSOME_II:RNAi
Sequence_infoPCR_productsjj_W03C9.4
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneW03C9.4gInferred_automaticallyRNAi_primary
W03C9.4hInferred_automaticallyRNAi_primary
W03C9.4eInferred_automaticallyRNAi_primary
W03C9.4dInferred_automaticallyRNAi_primary
W03C9.4iInferred_automaticallyRNAi_primary
W03C9.4bInferred_automaticallyRNAi_primary
W03C9.4fInferred_automaticallyRNAi_primary
W03C9.4aInferred_automaticallyRNAi_primary
GeneWBGene00003015Inferred_automaticallyRNAi_primary
TranscriptW03C9.4g.1Inferred_automaticallyRNAi_primary
W03C9.4h.1Inferred_automaticallyRNAi_primary
W03C9.4b.1Inferred_automaticallyRNAi_primary
W03C9.4e.1Inferred_automaticallyRNAi_primary
W03C9.4d.1Inferred_automaticallyRNAi_primary
W03C9.4a.1Inferred_automaticallyRNAi_primary
W03C9.4f.1Inferred_automaticallyRNAi_primary
W03C9.4i.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
Phenotype_not_observedWBPhenotype:0000961RemarkRNAi did not affect expression of the fkh-6::GFP transgene in hermaphrodite animals
Remark(Table S1) lin-29 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi