WormBase Tree Display for RNAi: WBRNAi00097373

WBRNAi00097373 Homol: Homol_homol: F54G8:RNAi
  Sequence_info: PCR_product: sjj_F54G8.3
  Experiment: Laboratory: XF
    Date: 08 May 2006 00:00:00
    Treatment: Animals were grown in liquid RNAi cultures for 2 days and then irradiated at 120 Gy on NGM plates with corresponding RNAi foods by using a Gammacell 1000 (Cs-137) irradiator with a dose rate of 6 Gy/min. For observation of germline apoptosis, the worms were soaked in acridine orange (1% v/v) in M9 for 4 hours, 20 hours after irradiation. After washing and 1 hour recovery on bacterial food, the number of apoptotic bodies was quantified with a Nomarski microscope.
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: F54G8.3 Inferred_automatically: RNAi_primary
    Gene: WBGene00002081 Inferred_automatically: RNAi_primary
    Transcript: F54G8.3.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00027740
  Phenotype: WBPhenotype:0000184 Remark: RNAi results in a decreased number of apoptotic bodies in the "apoptotic zone" of the germ line after irradiation compared to wild type, suggesting that germ cells are allowed to proliferate even in the presence of DNA damage.
    WBPhenotype:0000712 Remark: RNAi results in a decreased number of apoptotic bodies in the "apoptotic zone" of the germ line after irradiation compared to wild type, suggesting that germ cells are allowed to proliferate even in the presence of DNA damage.
  Remark: (Figure 1E) ina-1 RNAi
  Method: RNAi