WormBase Tree Display for RNAi: WBRNAi00097301
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WBRNAi00097301 | Homol | Homol_homol | C49H3:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_C49H3.10 | |||
Experiment | Laboratory | XF | |||
Date | 08 May 2006 00:00:00 | ||||
Treatment | Synchronized wild-type L1 worms were grown in liquid culture with bacteria expressing dsRNA for 2 days. L4 animals were exposed to 0.14 mM camptothecin (Sigma) in 0.5% DMSO in M9 by soaking for 2 hours. Control animals were mock-treated with 0.5% DMSO. After treatment, the animals were transferred to plates with the original RNAi food. Twenty-four hours later four sets of three worms were transferred to a second plate to allow egg laying. The parents were removed after 18 hr. Dead eggs and offspring were counted 24 hr thereafter. | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | C49H3.10c | Inferred_automatically | RNAi_primary | |
C49H3.10a | Inferred_automatically | RNAi_primary | |||
C49H3.10b | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00002080 | Inferred_automatically | RNAi_primary | ||
Transcript | C49H3.10b.1 | Inferred_automatically | RNAi_primary | ||
C49H3.10c.1 | Inferred_automatically | RNAi_primary | |||
C49H3.10a.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00027740 | ||||
Phenotype_not_observed | WBPhenotype:0001918 | Remark | RNAi did not result in hypersensitivity to the drug camptothecin | ||
Affected_by | Molecule | WBMol:00001694 | |||
Remark | (Table 1) C49H3.10/imb-6 RNAi | ||||
Method | RNAi |