WormBase Tree Display for RNAi: WBRNAi00097266
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WBRNAi00097266 | Homol | Homol_homol | M04F3:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_M04F3.1 | |||
Experiment | Laboratory | XF | |||
Date | 08 May 2006 00:00:00 | ||||
Treatment | Synchronized wild-type L1 worms were grown in liquid culture with bacteria expressing dsRNA for 2 days. L4 animals were exposed to 0.14 mM camptothecin (Sigma) in 0.5% DMSO in M9 by soaking for 2 hours. Control animals were mock-treated with 0.5% DMSO. After treatment, the animals were transferred to plates with the original RNAi food. Twenty-four hours later four sets of three worms were transferred to a second plate to allow egg laying. The parents were removed after 18 hr. Dead eggs and offspring were counted 24 hr thereafter. | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | M04F3.1 | Inferred_automatically | RNAi_primary | |
Gene | WBGene00019767 | Inferred_automatically | RNAi_primary | ||
Transcript | M04F3.1.1 | Inferred_automatically | RNAi_primary | ||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00027740 | ||||
Phenotype | WBPhenotype:0001918 | Remark | RNAi resulted in hypersensitivity to the drug camptothecin | ||
Affected_by | Molecule | WBMol:00001694 | |||
Remark | (Table 1) M04F3.1 RNAi | ||||
Method | RNAi |