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WormBase Tree Display for RNAi: WBRNAi00097266

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Name Class

WBRNAi00097266HomolHomol_homolM04F3:RNAi
Sequence_infoPCR_productsjj_M04F3.1
ExperimentLaboratoryXF
Date08 May 2006 00:00:00
TreatmentSynchronized wild-type L1 worms were grown in liquid culture with bacteria expressing dsRNA for 2 days. L4 animals were exposed to 0.14 mM camptothecin (Sigma) in 0.5% DMSO in M9 by soaking for 2 hours. Control animals were mock-treated with 0.5% DMSO. After treatment, the animals were transferred to plates with the original RNAi food. Twenty-four hours later four sets of three worms were transferred to a second plate to allow egg laying. The parents were removed after 18 hr. Dead eggs and offspring were counted 24 hr thereafter.
Delivered_byBacterial_feeding
InhibitsPredicted_geneM04F3.1Inferred_automaticallyRNAi_primary
GeneWBGene00019767Inferred_automaticallyRNAi_primary
TranscriptM04F3.1.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00027740
PhenotypeWBPhenotype:0001918RemarkRNAi resulted in hypersensitivity to the drug camptothecin
Affected_byMoleculeWBMol:00001694
Remark(Table 1) M04F3.1 RNAi
MethodRNAi