WormBase Tree Display for RNAi: WBRNAi00097232
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| WBRNAi00097232 | Homol | Homol_homol | T27C4:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_T27C4.4 | |||
| Experiment | Laboratory | XF | |||
| Date | 08 May 2006 00:00:00 | ||||
| Treatment | Synchronized wild-type L1 worms were grown in liquid culture with bacteria expressing dsRNA for 2 days. L4 animals were exposed to 0.3 mM cis-diamminedichloroplatinum (cisplatin) (Platosin dissolved in 0.9% NaCl, Pharmachemie BV) in M9 for 3 hr. Control animals were mock-treated with 0.9% NaCl in M9. After treatment, the animals were transferred to plates with the original RNAi food. Twenty-four hours later four sets of three worms were transferred to a second plate to allow egg laying. The parents were removed after 18 hr. Dead eggs and offspring were counted 24 hr thereafter. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | T27C4.4e | Inferred_automatically | RNAi_primary | |
| T27C4.4b | Inferred_automatically | RNAi_primary | |||
| T27C4.4d | Inferred_automatically | RNAi_primary | |||
| T27C4.4a | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00003025 | Inferred_automatically | RNAi_primary | ||
| Transcript | T27C4.4d.1 | Inferred_automatically | RNAi_primary | ||
| T27C4.4b.2 | Inferred_automatically | RNAi_primary | |||
| T27C4.4a.1 | Inferred_automatically | RNAi_primary | |||
| T27C4.4e.1 | Inferred_automatically | RNAi_primary | |||
| T27C4.4b.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00027740 | ||||
| Phenotype_not_observed | WBPhenotype:0001918 | Remark | RNAi did not result in hypersensitivity to the drug cisplatin | ||
| Affected_by | Molecule | WBMol:00002867 | |||
| Remark | (Table 1) T27C4.4/egr-1 RNAi | ||||
| Method | RNAi |
