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WormBase Tree Display for RNAi: WBRNAi00096853

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Name Class

WBRNAi00096853HomolHomol_homolT21E12:RNAi
Sequence_infoPCR_productsjj_T21E12.4
ExperimentLaboratoryGR
Date23 Feb 2012 00:00:00
TreatmentSynchronized L1s were grown on aversion-inducing RNAi bacteria or control RNAi bacteria for 48-54 hours prior to conducting behavioral assays. Animals were tested for attraction to a spot of 1 microliter isoamyl alcohol on fresh 10 cm NGM plates. Chemotaxis was measured after 1 hour and calculated using the standard formula (CI = [# of animals on odorant-# of animals on control]/[total number of animals]). All assays were performed in triplicate in at least 3 independent trials. Approximately 50-100 animals were tested per assay plate.
Delivered_byBacterial_feeding
InhibitsPredicted_geneT21E12.4bInferred_automaticallyRNAi_primary
T21E12.4aInferred_automaticallyRNAi_primary
GeneWBGene00000962Inferred_automaticallyRNAi_primary
TranscriptT21E12.4a.1Inferred_automaticallyRNAi_primary
T21E12.4b.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00040984
Phenotype_not_observedWBPhenotype:0000304RemarkRNAi did not result in defective chemotaxis to isoamyl alcohol
Remark(Figure S7A) dhc-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
MethodRNAi