WormBase Tree Display for RNAi: WBRNAi00094577
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| WBRNAi00094577 | Homol | Homol_homol | ZK381:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_ZK381.1 | |||
| Experiment | Laboratory | KLJ | |||
| Date | 07 Jul 2009 00:00:00 | ||||
| Strain | WBStrain00000001 | ||||
| Treatment | L4 hermaphrodites were put on RNAi and control plates for 36 h. The worms were then transferred to fresh RNAi and control plates, respectively. After 24 h, the parental worms were removed and the progeny were allowed to develop. The L4 hermaphrodites of RNAi-treated progeny were collected for experiments. These animals were on RNAi during development. RNAi-treated L4 stage nematodes were transferred to NGM agar plates containing 100 microliters of Salmonella typhimurium (strain ATCC14028s) that had been cultured overnight in LB broth and incubated on plates for 6 hours at room temperature. After 48 hours of infection, animals were transferred to fresh feeding RNAi or vector-control plates, and survival was scored daily. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | ZK381.1a | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00001862 | Inferred_automatically | RNAi_primary | ||
| Transcript | ZK381.1a.1 | Inferred_automatically | RNAi_primary | ||
| ZK381.1b | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00035076 | ||||
| Phenotype_not_observed | WBPhenotype:0001271 | Remark | him-3 RNAi does not result in a significantly shorter lifespan in the presence of Salmonella typhimurium bacteria, compared to vector controls | ||
| Remark | (Figure S1A, Table S2) him-3 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | ||||
| Method | RNAi |
