WormBase Tree Display for RNAi: WBRNAi00088936
expand all nodes | collapse all nodes | view schema
| WBRNAi00088936 | Homol | Homol_homol | F28B3:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | ATGCGTGGCGGAAGCTCGCTGGATAGTTTTCCAGGCAAAGGAACTCTCCACACACTCGAGATCGAAAACTTCAAATCTTACAAGGGAAAACATACTATTGGACCCTTTACAAGATTCACAGCTATCATTGGACCAAATGGTTCAGGAAAATCAAATCTTATGGACGCAATTTCGTTCGTGCTCGGAGAGAAGCCAAGCAGTTTGCGTGTTAGAAAGTATGCGgtaagagaaaaagtataaaatatagaagattttaatcaataatgtttagGATTTGATTCATGGTGCACCAATTAACAAGCCAGTTGCTAAGAAATGTCGTGTCACTATGAACTACAAATATTCTGACGGAAAAGTCAAGGCATTCACCAGAGGCGTCAATAATGGAACATCTGAACATCTTCTTGATGGTCAAACTGTCACAAGTGCAGCATATTCACAAGAAATGGAAAGTATCAATATCTTCATCAAGGCAAGAAACTTTCTGGTATATCAGGGAGCTATCGAAAATATTGCCATGAAAACTCCAAAAGAACGTACTCAACTCTTCGAGGAGCTCTCACGATCACACGAGTTTCAAGCAGAGTATGAAAGATTGAAAGTTGAGATGACAAAAGCTGAAGATGATACTCAACACAATATGAACAAGAGACGAGGAATCGCTCAAGAGAAACGTGAGGCAAAAATGGAGAAAGATGAAGCTGAAAAGTATCAAACCATGAAGAATGAGCTGGCTGCAAAATCGACGATGCTATTTCTCCATCAATTGTTCCATTGCGAACGGACGATCGACGAATCAAAAGAGGAGATCAACGCTCAGAAAAAGACGATTGCAAGTCTCGAAGCAACTAGGgtatgatgaaggcaattctactaaatgacgttcttccaatatttttcattcatattttgtgataaactttagaaaaatattttctttatctgaaaattacttatttaacttggaacaacttaaaaagcagttcttccaagttatacaaaagttattttccggtgaagctaatattgttcaatattttatcgtaaaattttgattgattgatttatagtatataaaaataatcaaaattatatattacagTCGAAAGAAGAAGCGAAAATCGCTGCGGTTCATCAGGAGCACCGGAAAGCTCTCCGCGAGGTTCAGAAGATGACAAGAAAACTCGATCAGAAAGAGACGGATTTGGCTGAAAAGCAACAAAATATGCTGACATTGAAAGTTTCCGTTGCTCACGAGCACAAGAAGCTGGAAATTGCGAAGAAGATGCTTGCCGCTGCAGAAAGTAAGGCAGAGAACAACTCGACTCAGCTGGCGGATTTGAAGAAAAGCAAAAAAGAGCTAGAAAAGAAGAAGGCCGCATATGAAGCAGAGATTCAGGATATGATGCAAAGAGGCGAGTTAAATCTTTCGGATGAACAAGTACGTGAGTACGGACAGCTGAAAGATCAAGCTCAGCGAGAATCAGCAATGGTTCAAAGAGAGCTATTGATGGCCGAACAAGTGTTCGAAGGTGATAAGAGTTCTCTAAATCATGAGCTTCGACGCCAAAAGGAGCATCAAGAAAGAGTTAAAGCGAAGGAAGGTGATGTGCGAAGAATTGAAACTCAAATTGCAACTCTTGCTCAAAGAATCAAGGAAACTGAAGAAGAGACAAAGATCTTGAAAGCTGATCTTAAGAAGATTGAAAATGACGTTGTTATTGATAAATCAGCAGCAGCGGAATACAACAAAGAGCTTGTAGCTGTCGTTCGTCAACTCTCCGAAGCTTCTGGAGATTCTGCAGAAGGAGAAAGAAATCAAAGAAGAACGGAGGCACTTGAAGGATTGAAAAAGAATTTCCCGGAGAGTGTGTATGGTAGACTTGTCGATCTCTGTCAACCGTCTCATAAACGGTTCAACATTGCCACCACAAAGATTCTTCAGAAGCATATGAACAGTATTGTTTGTGATACTGAAGAGACTGCTGCCAAGGCAATTGTTTATCTGAAGgtttgttcttttttcacttccaaaatttcaattcataataactaatttccagGATCACCGTTATCCCCCAGAAACCTTCCTTCCAAATGATGCACTAGTTGTTAATCCACTAAATGAAAAACTTCGTGAAATCAAAAAGCCAGCTGGAGTGAAGCTCGTCTTCGATGTCATCAATCCTCAACATCAAGCAGCTAGAAAAGCTCTTCAATTCGTTTGTGGAAACGCATTAGTTTGTGAGAGTCAAGAAGATGCTAAGCAGCTTGCATACGGTGGAGGAGAGCTGAAAGATCGTTTCAAGGCAGTTTCGATGGACGGAACTCTGTTCCAACAATCTGGAGTTATGTCCGGAGGTTCTGCTGATTTGAGACAAAAGTCCAAGAAATGGGACGAGAAAGTTGTCAAgtgagtttttattttattttttattaaattgaaaaaaaaacattaatatttttccagACAACTCCGAGAAAAGCGGAACCAACTGAACGAAAAAATCGCGGATCTTCAGAAACATCGTCGTCGTGAACTCGAAGTGGAAAGTGTACGCTCCAAGATTAATGGAAATGAGCAAAGATTGGCAATGATGAAGCGAGATCTGAAGAATATGCGAGAAATGCAGTTGGAAAGACTTCAAAATGAGCTTGAAGGCATGACCGCGGAGATGAATATGCTTCCGgtgattatttttagtgaccaacggttctggaaaaaaaaaattataaccccaaaaagtgctcaacttaacgaatttcgtaatgaaaattctcaaaaatctttcaaaaatttatttattttggagcaaattttggagccattagacctccttttaaatttgtaatctgcaaaaattgatatattttcagCCGCGAATCTCGAATTGTCAAGAAAAACTTGAACGCAGTGAAAGTACTTTAAAATCTCTGCAAACGAAGAGCAATGAAGTGGCTGATCGAATTTTCGCTGATTTCTGTACTCGTGTTGGAATCGCATCGATTCGTGACTATGAGAATCGTGAAATGCGTATCAAGCAAGAAATGGAAGACAAGCTTCGATCATTTGACGATGATATCCAAAAGTTGGCCTACGAAATTGACTTTGTCACCGAGCAAGATGGCAATCGGAAAGTTGAAGTTGAAAAGGAGAAAgtacgtttttcatttttttgtaaagaaactccttatcagtttctttaagattcttaaaattgttttgtttaccttatttgaaatatttttattacagGTTTCACAAATCGACCGACAATATAAGGACATGAAGAAAAAAGAGAAAACGGCGGCGGCCGCTTTGAAAGAGCACACGGAATCCATGGAGCAAGACAAGGAAGTGCTGGAAGAGAAGAAGGCCTTATCACATAAATTGGAAACCGAGTGGAATGAGGTCAAAAAAATTGCACAAGTTGCGATGAAAGACTTTACAAAGGCTGAAAAAGAGCTTCTTCGTCTTGAAAGTTTGCTTACTAAGAAGCAATATGAACGGCACTCTCTTCTTCATTCTGTGAAGCTTGGGCAAATTGCGTTACCACTCAAATCAGGAAGTATGGCTGACGTTGAATATGAGGAAGATGATGGAGgttggatatttattttgattttgatgttttgaaaaaatcgcattttcacttttccataattcagttttcagcttttagatatatatttcataatttttgtctccctgtttcctttcaagttccaattaatttcagATGACACAGCTTCTCAGTCTTCTCAATCAGCCACCGATGGTCCAAGTGTATCAGAGGAGCAAATTCAACGAGAGCAACACATCAAAATCAACTACGACTCGTTACCGCGAGAATATAAAGATgttagtgctgccaagacatgtttcggttttctgagcaaaccgaaaaaatactggcaacaaaaatttatttattttttctgaactattttgaccctaaattttttcgatttttgagccaaacaaaatacgaaaaaaatctactttttcggtttaaaaaaaaccagcagtcctgttagtgatggttgctgatcaaaaattatttgctcttttgccaaccccgccccgccctgcccccctccgtgttttagttcatttctagattcacacgatttgcattacagGTGGATGACGATGATGGTGTACGGCAAATGTCGAATCGACTCAATGTGGAAATCGATGAACTGCAGAAAAATGTGTCAAAAATGAATGCGCCGAATTTGAAAGCCAATCAGAGAATGGCAGAGGTGAAAGAACGAGAAGCCGAGTCGACTGAAGAACTTGAAAATGCTCGAAAGAAGGCGAAGAGAATTCGGCAACAGTTCGAGAAGgttagttattaagaaattccaaacgtttagtatctgaaaatgtatcgataaaaatgaaagtttaggagagcttcgaatccattttagacattgaaaaatttagattttacacataattttgtgggccaaaataaaaaaaattccttgactagataaaaactagaaaattagctaaaaaatatatatttaaaacctacaaaatgcattattttttaaatgtcaaaaatggattcaaatcaggagtgggcatcagttattttttcaaagtatagaaaatctcttgatttatctgaaaagaacatttatttcagtcaaaagatttgataaagtactcaaaaaatgttattttcacagatatcgggtgattttttacattttgacattttttaaattaaaaacgttgaaccaacatgtttgaatcaccgaaaaaaaaaccgcttcattaaaataatttttttgcgataattttcctatactgaaaataagaatattcttgaaattccttgaaatgaaaatgtgcaccttctggacaccaagtgacaaaaacattagagataagagacatagacattcataactttaaaatgacaacagttgtacccatattgtcactcaatctgtcccctctatctctcacacctcttcaatttcacttttctcatatattttatcgattttaaaatattgtttttttcagGTAAAAACCGATCGCTACCGACGCTTCCAAGATTTCTTCGATCCTGTGGCCAACACTATCGATGACATTTACAAGCAACTCTCACGAAATACTTCTGCTCAAGCCTTCCTCGGAGCTGATAACATGGAAGAGCCCTATCTGGATGGTATCCAGTACAATTGTGTAGCTCCTGGAAAACGTTTCCGTCCGATGGATAACTTGTCTGGAGGAGAAAAGACAATTGCCGCGTTGGCACTGCTTTTTGCAGTTCACGGACGTAATCCTGCTCCGTTTTTCGTTCTCGACGAGATTGATGCTGCTTTGGATAATACTAATATTGGAAAGgtatttatttaaactgttcattaaaattaaattttgcttcaaaagatagaaaaatgactgtaatgaccgaatattactgttgtaaaacacaaaaattataaaataagggtaaatcctccggtttttctgcatatttttgatagttgatctaaaaaactatcaaactatcaaacttttaagcaaaatttcaaaacgattttactccgcctttagtataacctgcgagaaaagattgtaacgttttttttgtaatttcagGTCGCTTCGTACATCTGTGAATCAGCTCGTGAGCATATGCAAATTATCGTCATTTCTCTCAAAGAAGAGTTCTACAACAAAGCTGACTCACTCATCGGAATCTTCCCATATCCAGCCGCTTGTACAACATCTGGTGTTCTCACTTTCGATTTGACGCGCTTCAAACAAATTGGTCTCAATGAAATGACTGAAAATCCACCAACACCATCGATTGCAACATAA | |||
| Experiment | Laboratory | TY | |||
| Date | 26 Jun 2003 00:00:00 | ||||
| Delivered_by | Injection | ||||
| Inhibits | Predicted_gene | F28B3.7a | Inferred_automatically | RNAi_primary | |
| F28B3.7b | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00001860 | Inferred_automatically | RNAi_primary | ||
| Transcript | F28B3.7b.1 | Inferred_automatically | RNAi_primary | ||
| F28B3.7a.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00005919 | ||||
| Phenotype | WBPhenotype:0000773 | Remark | Nuclei from embryos treated with double RNAi against him-1 and either scc-1 or tim-1 had numerous FISH signals, indicating severe chromosome segregation defects and extensive aneuploidy. | ||
| Remark | paper remark: Exact sequence used for RNAi not stated by authors, genomic sequence of gene used for curation. Primers used were: him-1 5' GCGTAATACGACTCACTATAGGGAAGGCAGAGAACAACTCGACTCAG 3' and 5' CGCAATTAACCCTCACTAAAGGGATCAGCAGAACCTCCGGACATA 3', tim-1 5' GCGTAATACGACTCACTATAGGGCGAGATTTAATTCGATATCTACGCGA 3' and 5' CGCAATTAACCCTCACTAAAGGGCTTCAGAATTTGAAGTACAAGGCCC 3'. | ||||
| Method | RNAi |
