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WormBase Tree Display for RNAi: WBRNAi00086040

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Name Class

WBRNAi00086040HomolHomol_homolY55D5A:RNAi
Sequence_infoPCR_productsjj_Y55D5A_391.b
ExperimentLaboratoryCT
Date04 Nov 2010 00:00:00
Genotypemir-71(n4115)
TreatmentSynchronized L1s were fed with OP50, grown to young adults, and transferred to RNAi expressing HT115 (DE3) bacteria plates containing (FUDR, 0.1g/ml) to prevent progeny production. Fresh, IPTG-induced RNAi-expressing bacteria were plated onto RNAi plates every 5-7 days. Life span was assessed every 1-2 days.
Delivered_byBacterial_feeding
InhibitsPredicted_geneY55D5A.5eInferred_automaticallyRNAi_primary
Y55D5A.5cInferred_automaticallyRNAi_primary
Y55D5A.5dInferred_automaticallyRNAi_primary
Y55D5A.5aInferred_automaticallyRNAi_primary
GeneWBGene00000898Inferred_automaticallyRNAi_primary
TranscriptY55D5A.5e.1Inferred_automaticallyRNAi_primary
Y55D5A.5bInferred_automaticallyRNAi_primary
Y55D5A.5a.1Inferred_automaticallyRNAi_primary
Y55D5A.5c.1Inferred_automaticallyRNAi_primary
Y55D5A.5d.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000500578
ReferenceWBPaper00037855
PhenotypeWBPhenotype:0000039Remarkmir-71(n4115) animals grown on daf-2 (RNAi) were shorter lived than wild-type animals grown on daf-2(RNAi), demonstrating that loss of miR-71 partially suppresses the long life span induced by daf-2(RNAi).
RemarkExact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi