WormBase Tree Display for RNAi: WBRNAi00086040
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WBRNAi00086040 | Homol | Homol_homol | Y55D5A:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_Y55D5A_391.b | |||
Experiment | Laboratory | CT | |||
Date | 04 Nov 2010 00:00:00 | ||||
Genotype | mir-71(n4115) | ||||
Treatment | Synchronized L1s were fed with OP50, grown to young adults, and transferred to RNAi expressing HT115 (DE3) bacteria plates containing (FUDR, 0.1g/ml) to prevent progeny production. Fresh, IPTG-induced RNAi-expressing bacteria were plated onto RNAi plates every 5-7 days. Life span was assessed every 1-2 days. | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | Y55D5A.5e | Inferred_automatically | RNAi_primary | |
Y55D5A.5c | Inferred_automatically | RNAi_primary | |||
Y55D5A.5d | Inferred_automatically | RNAi_primary | |||
Y55D5A.5a | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00000898 | Inferred_automatically | RNAi_primary | ||
Transcript | Y55D5A.5e.1 | Inferred_automatically | RNAi_primary | ||
Y55D5A.5b | Inferred_automatically | RNAi_primary | |||
Y55D5A.5a.1 | Inferred_automatically | RNAi_primary | |||
Y55D5A.5c.1 | Inferred_automatically | RNAi_primary | |||
Y55D5A.5d.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Interaction | WBInteraction000500578 | ||||
Reference | WBPaper00037855 | ||||
Phenotype | WBPhenotype:0000039 | Remark | mir-71(n4115) animals grown on daf-2 (RNAi) were shorter lived than wild-type animals grown on daf-2(RNAi), demonstrating that loss of miR-71 partially suppresses the long life span induced by daf-2(RNAi). | ||
Remark | Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | ||||
Method | RNAi |