WormBase Tree Display for RNAi: WBRNAi00084975
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WBRNAi00084975 | Homol | Homol_homol | F54D8:RNAi | |||
---|---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_F54D8.6 | ||||
Experiment | Laboratory | RZ | ||||
Date | 19 Nov 2010 00:00:00 | |||||
Genotype | otIs138 | |||||
Treatment | Three L4 larval stage hermaphrodites were placed on each RNAi plate, incubated for 4 days at 20C and allowed to lay a brood. After incubation, young adult progeny were washed off feeding pates with M9 buffer and mounted on 5% agar pads. Approximately twenty adult progeny per RNAi clone were scored for the presence of defective PVD dendritic morphology, defined here as a qualitative increase or decrease in the number of secondary, tertiary or quaternary dendrites. | |||||
Temperature | 20 | |||||
Delivered_by | Bacterial_feeding | |||||
Inhibits | Predicted_gene | F54D8.6 | Inferred_automatically | RNAi_primary | ||
Gene | WBGene00018802 | Inferred_automatically | RNAi_primary | |||
Transcript | F54D8.6.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | |||||
Reference | WBPaper00037977 | |||||
Phenotype | WBPhenotype:0000882 | Remark | Animals exhibited a dendrite branching defect in PVD neurons | |||
Penetrance | Range | 20 | ||||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
Remark | Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | |||||
Method | RNAi |