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WormBase Tree Display for RNAi: WBRNAi00079862

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Name Class

WBRNAi00079862HomolHomol_homolC32D5:RNAi
Sequence_infoDNA_textagaaaacatgattttctgggaggggagaagagcaacttcgtagacaatagtttgaatagaagtcactactcggaaccgtcatacgagaaaaatgagaagaaaatgtgaaggaaatacagaattattacagaaaatgagaagatttttttaaaggggctttgggtttccattagaagtatgaaatttccattgtacaagtatacacattcgtcggcggataatacatgacactttattccttcttttcgacctctcctccatacacactttcgtcactgtaggcgatgtaaaggaacaagtcttcctcgtgatggtcctgtaataaagaaaagggttaattaaatgaaaataaacgactggttagttacctggtagagttgtcccattgtggtcatggtttgtggaatgacattgttgacaaagaagaacagagcatcttctggacgaagttggatgcgttttctgatgaggaagtagaactgtccaacagtaagatcggatgggaccaagtacttcttcttatccaagtcatggagctttgactttggtgctttctcaacaatcactggaatacggtctgggtactttctgcggatcttgtctccttcggcacgacgcttctcaaagttgttctcctccttgtaagcccacttcattttgattcgaaggttagtgtgaa
ExperimentDate28 Jul 2003 00:00:00
Genotypedaf-2(e1370)
Temperature25
Delivered_byInjection
InhibitsPredicted_geneC32D5.9Inferred_automaticallyRNAi_primary
GeneWBGene00002980Inferred_automaticallyRNAi_primary
TranscriptC32D5.9.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00006070
PhenotypeWBPhenotype:0000308RemarkKnocked down gene not required for dauer initiation but is required for normal dauer morphogenesis in daf-2(e1370) mutant animals.
WBPhenotype:0001545RemarkAnimals formed abnormal dauers. Like the daf-2(e1370) dauers, these animals underwent a developmental arrest in the L3 gonadal stage and had some hyperpigmented granules in the intestine, some increased fat storage, and some radial constriction and elongation of the body and pharynx. However, the hyperpigmented granules were unevenly distributed and the magnitudes of fat storage, radial constriction, and elongation of the body and pharynx were less than that observed in untreated daf-2(e1370) animals. Furthermore, animals failed to form dauer alae, were not resistant to SDS, died within a few days at 25C, and usually did not resume reproductive growth on transfer to 15C.
MethodRNAi