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WBPicture0000014627DescriptionFig 8. ngat-1p::gfp transcriptional reporter expression pattern. A chromosomally integrated ngat-1p::gfptranscriptional reporter strain was made as described in Materials and methods. A dpy-7p::mCherrytranscriptional reporter was injected into the ngat-1::gfp integrated line to create a non-integrated marker forepidermal cells (see Materials and methods), which may not mark all epidermal cells in a given animalbecause of potential mitotic loss of the extrachromosomal array. (A-B) merge of gfp and mCherry signalsshowing presumptive mCherry-expressing epidermal cells (thick arrows) that co-express gfp (as indicated byorange tinge) plus presumptive muscle cells (thin arrows), and presumptive intestinal cells (star) that expressonly ngat-1p::gfp in a comma stage embryo. (C-E) The ngat-1p::gfp reporter is expressed in body wallmuscles (arrows in C and E) and in lateral epidermis (asterisks in D and E) in all post-embryonic larval stages.Some unidentified elongated cells in the head also express gfp and at least one (arrowhead) also expressesmCherry. (Dark oval-shaped areas in the lateral epidermis are lateral seam cells). rf = red fluorescencechannel; gf = green fluorescence channel. Scale bar in panel A (for A and B) is 12.5 um. Scale bar in panel C(for C-E) is 100 um.
NameFigY.jpg
DepictExpr_patternExpr13499
AnatomyWBbt:0005733
WBbt:0005739
WBbt:0005753
WBbt:0005772
WBbt:0005792
WBbt:0005813
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
Publication_year2017
Article_URLDOIid10.1371/journal.pone.0183049
Journal_URLPLoSOne
Publisher_URLPLoS
ReferenceWBPaper00052947